EFFECT OF INCUBATION PERIOD AND GROWTH MEDIUM COMPOSITION ON EXOPOLYSACCHARIDES YIELD WITHIN INDIGENOUS FERMENTED KENYAN MURSIK MILK
EFFECT OF INCUBATION PERIOD AND GROWTH MEDIUM COMPOSITION ON EXOPOLYSACCHARIDES YIELD WITHIN INDIGENOUS FERMENTED KENYAN MURSIK MILK
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孵化期和生长培养基成分对本土发酵肯尼亚 MURSIK 奶中胞外多糖产量的影响
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
P. Muliro
中科院分区：
文献类型：
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作者：
M. Sc;A. Shitandi;P. Muliro
研究了培养时间和培养基对肯尼亚传统发酵乳胞外多糖产量的影响，以确定产胞外多糖的最佳条件。在20 ± 1 ℃下，在四种不同的生长培养基中监测5天的EPS产量水平：重构脱脂乳（RSM）、含5%蔗糖的RSM（RSM-S）、乳清（WH）和含5%蔗糖的WH（WH-S）。该研究确定，传统Mursik中EPS生产的最佳孵育期为4天后，平均产量为73 mg/L Gluc。当量不同培养基中EPS含量差异显著（p WHS>WH>RSM）。除18小时和24小时外，所有孵育期的平均EPS水平差异显著（p 18小时> 30小时> 12小时> 6小时）。产生最大EPS水平（141.5 mg/L Glu.当量）为RSM-S/ 24小时。得出的结论是，四天的孵育期是最佳的EPS水平在传统的穆尔西克。
The effect of incubation period and growth medium on Exopolysaccharides (EPS) yield from a traditionally fermented Kenyan milk (Mursik) culture was studied to determine the optimum conditions for EPS production. EPS yield levels were monitored over a 5 day period in four different growth media as: Reconstituted skim milk (RSM), RSM with 5% sucrose (RSM-S), Whey (WH) and WH with 5% sucrose (WH-S) at 20 ± 1oC. The study established that the optimum incubation period for EPS production in traditional Mursik occurred after 4 days with an average yield of 73 mg/L Gluc. Equiv. The mean EPS level in all the growth media differed significantly (p WHS>WH>RSM. Also, except for 18 and 24 hrs, the mean EPS levels for all the incubation periods differed significantly (p 18hrs>30hrs >12hrs >6hrs. The growth medium/incubation period interaction which yielded maximum EPS level (141.5 mg/L Glu. equiv) from Mursik culture was RSM-S/ 24 hrs. It was concluded that a four day incubation period was optimal for EPS levels in traditional Mursik.