Anatomy of the posterior caudal lobe of the cerebellum and the eminentia granularis posterior in a mormyrid fish
Anatomy of the posterior caudal lobe of the cerebellum and the eminentia granularis posterior in a mormyrid fish
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DOI：
10.1002/cne.21334
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发表时间：
2007-06-10
影响因子：
2.5
通讯作者：
Bell, Curtis C.
中科院分区：
文献类型：
--
作者：
Campbell, Holly R.;Meek, Johannes;Bell, Curtis C.
长颌鱼的小脑因其巨大的体积和独特的组织结构而引起人们的兴趣。像所有鳍鱼一样，长颌鱼的小脑有三个亚部：瓣部、体部和尾叶。以前已经检查过莫米里德瓣和体的结构，但尚未研究莫米里德尾叶的结构。莫米里德尾叶包括与电感觉相关的后尾叶和与侧线和第八神经感觉相关的前尾叶。在这篇文章中，我们描述了后尾叶和隆起的颗粒后（EGp）的细胞成分，在长颌鱼Gnathonemus petersii。EGp产生后尾叶的平行纤维。我们使用细胞内注射的生物胞素，细胞外注射的生物素化葡聚糖胺，和免疫组化抗体的γ-氨基丁酸，肌醇三磷酸受体I，钙视网膜蛋白，和ZEEPRONII。后尾叶的组织学结构明显不规则，与体和瓣相比，小脑成分的紧密模块化组织不太明显。大多数浦肯野细胞体位于分子区域的中间。其树突在矢状面上仅大致定向，向腹侧和背侧延伸，分支不规则。攀缘纤维仅在索马附近的光滑树突上终止。大多数浦肯野细胞轴突终止于皮质外的广枝细胞。这些结果为已经存在的大量不同小脑和小脑样结构提供了一个额外的变体。
The cerebellum of mormyrid fish is of interest for its large size and unusual histology. The mormyrid cerebellum, as in all ray-finned fishes, has three subdivisions-valvula, corpus, and caudal lobe. The structures of the mormyrid valvula and corpus have been examined previously, but the structure of the mormyrid caudal lobe has not been studied. The mormyrid caudal lobe includes a posterior caudal lobe associated with the electrosense and an anterior caudal lobe associated with lateral line and eighth nerve senses. In this article we describe cellular elements of the posterior caudal lobe and of the eminentia granularis posterior (EGp) in the mormyrid fish Gnathonemus petersii. The EGp gives rise to the parallel fibers of the posterior caudal lobe. We used intracellular injection of biocytin, extracellular injection of biotinylated dextran amine, and immunohistochemistry with antibodies to gamma-aminobutyric acid, inositol triphosphate receptor I, calretinin, and Zebrin II. The histological structure of the posterior caudal lobe is markedly irregular in comparison to that of the corpus and the valvula, and a tight modular organization of cerebellar elements is less apparent here. Most Purkinje cell bodies are in the middle of the molecular region. Their dendrites are only roughly oriented in the sagittal plane, extend both ventrally and dorsally, and branch irregularly. Climbing fibers terminate only on smooth dendrites near the soma. Most Purkinje cell axons terminate locally on eurydendroid cells that project outside the cortex. The results provide an additional variant to the already large set of different cerebellar and cerebellum-like structures.