SEE locomotor behavior test discriminates C57BL/6J and DBA/2J mouse inbred strains across laboratories and protocol conditions
SEE locomotor behavior test discriminates C57BL/6J and DBA/2J mouse inbred strains across laboratories and protocol conditions
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DOI：
10.1037/0735-7044.117.3.464
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发表时间：
2003-06-01
影响因子：
1.9
通讯作者：
Golani, I
中科院分区：
文献类型：
--
作者：
Kafkafi, N;Lipkind, D;Golani, I
行为表型的常规测试通常难以区分某些基因型并在实验室和方案条件下复制这些差异。本研究探讨了这样一个假设，即自动化测试可以被设计成量化行为学相关的行为模式，更容易表征遗传和复制的表型。它使用SEE（探索的探索策略）在3个实验室中对C57 BL/6和DBA/2小鼠近交系的运动行为进行表型分析。这2种基因型在15种不同的行为指标上存在差异，但没有一种表现出显著的基因型-实验室交互作用。在同一实验室内，尽管试验光周期相位发生变化并注射生理盐水，但这些差异中的大多数在其他实验中得到了复制。结果表明，精心设计的测试可以大大提高跨实验室的可重复性。
Conventional tests of behavioral phenotyping frequently have difficulties differentiating certain genotypes and replicating these differences across laboratories and protocol conditions. This study explores the hypothesis that automated tests can be designed to quantify ethologically relevant behavior patterns that more readily characterize heritable and replicable phenotypes. It used SEE (Strategy for the Exploration of Exploration) to phenotype the locomotor behavior of the C57BL/6 and DBA/2 mouse inbred strains across 3 laboratories. The 2 genotypes differed in 15 different measures of behavior, none of which had a significant genotype-laboratory interaction. Within the same laboratory, most of these differences were replicated in additional experiments despite the test photoperiod phase being changed and saline being injected. Results suggest that well-designed tests may considerably enhance replicability across laboratories.