Chromatin regulation at the frontier of synthetic biology.
Chromatin regulation at the frontier of synthetic biology.
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DOI：
10.1038/nrg3900
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Collins JJ
中科院分区：
文献类型：
--
作者：
Keung AJ;Joung JK;Khalil AS;Collins JJ
随着合成生物学方法扩展到整个医学，生物技术和基础生物学研究的各种应用，越来越需要工程酵母，植物和哺乳动物细胞。真核生物的基因组受染色质的生物化学和生物物理状态的调控，这给在细菌中的应用带来了独特的挑战和机遇。最近的合成方法，包括“表观基因组编辑”，允许直接和功能解剖生理染色质调节的许多方面。这些研究为生物医学和生物技术工程应用奠定了基础，这些应用可以利用染色质调控的独特组合和时空层来创建前所未有的复杂合成系统。
As synthetic biology approaches are extended to diverse applications throughout medicine, biotechnology and basic biological research, there is an increasing need to engineer yeast, plant and mammalian cells. Eukaryotic genomes are regulated by the diverse biochemical and biophysical states of chromatin, which brings distinct challenges, as well as opportunities, over applications in bacteria. Recent synthetic approaches, including `epigenome editing', have allowed the direct and functional dissection of many aspects of physiological chromatin regulation. These studies lay the foundation for biomedical and biotechnological engineering applications that could take advantage of the unique combinatorial and spatiotemporal layers of chromatin regulation to create synthetic systems of unprecedented sophistication.