Rapid identification of species, sex and maturity by mass spectrometric analysis of animal faeces
Rapid identification of species, sex and maturity by mass spectrometric analysis of animal faeces
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DOI：
10.1186/s12915-019-0686-9
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发表时间：
2019-08-14
期刊：
影响因子：
5.4
通讯作者：
Beynon, Robert J.
中科院分区：
文献类型：
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作者：
Davidson, Nicola B.;Koch, Natalie, I;Beynon, Robert J.
我们描述了一种基于快速蒸发电离质谱（REIMS）产生的分子特征分析的粪便样本信息恢复的新方法。结果利用rems对5种不同鼠种的粪便颗粒进行了分析，并快速获得了复杂的质谱（每个样品通常在几秒内）。然后将未解释的质谱（特征）用于基于随机森林的线性判别分析和分类模型。无论粪便是来自标准实验室条件下饲养的动物还是野生捕获的动物，都有可能以很高的准确率对每个物种的来源进行分类。在一系列不同的条件下，REIMS特征对预先储存的粪便材料是稳定的，并且不会因饮食的变化而迅速或根本地改变。此外，在物种内，REIMS标记可用于区分成年小鼠与幼年小鼠，雄性小鼠与雌性小鼠以及来自三种不同实验室菌株的粪便。结论REIMS为粪便样本的快速分析提供了一种全新的方法，将粪便分析（以前主要集中在DNA上）扩展到表型评估，并且在野外生物学家的装备中具有相当大的潜力。
Background We describe a new approach to the recovery of information from faecal samples, based on the analysis of the molecular signature generated by rapid evaporative ionisation mass spectrometry (REIMS). Results Faecal pellets from five different rodent species were analysed by REIMS, and complex mass spectra were acquired rapidly (typically a few seconds per sample). The uninterpreted mass spectra (signatures) were then used to seed linear discriminant analysis and classification models based on random forests. It was possible to classify each species of origin with a high rate of accuracy, whether faeces were from animals maintained under standard laboratory conditions or wild-caught. REIMS signatures were stable to prior storage of the faecal material under a range of different conditions and were not altered rapidly or radically by changes in diet. Further, within species, REIMS signatures could be used to discriminate faeces from adult versus juvenile mice, male versus female mice and those from three different laboratory strains. Conclusions REIMS offers a completely novel method for the rapid analysis of faecal samples, extending faecal analysis (previously focused on DNA) to an assessment of phenotype, and has considerable potential as a new tool in the armamentarium of the field biologist.