Clustered integrin a5ß1 ligand displays model fibronectin-mediated adhesion of human endometrial stromal cells.
Clustered integrin a5ß1 ligand displays model fibronectin-mediated adhesion of human endometrial stromal cells.
复制标题
聚集的整合素 a5-1 配体展示了纤连蛋白介导的人子宫内膜基质细胞粘附模型。
DOI：
10.1016/j.bbrc.2011.03.099
复制
发表时间：
2011
影响因子：
3.1
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Li Z
由于不能模拟人子宫内膜间质细胞（EnSC）所需的纤连蛋白（FN）细胞外基质，子宫内膜组织工程用于模拟子宫内膜疾病和不孕症的进展受到挫折。在这里，我们表明，这是因为需要在特定方向的多价展示中呈现整合素α5β1（FN受体）配体;通过工程控制第9 - 10个III型FN结构域对（FIII 9 -10，最小整合素α5β1配体）的自组装，以特定方向固定在细胞培养物表面。对于多聚体配体表面，观察到粘附的EnSC的分散的分数显著增加，顺序为：四聚体>三聚体>二聚体>单体。EnSC扩散形态的程度也以相同的顺序增加，其中四聚体配体支持与FN支持的形态最相似的形态。我们的数据表明，只有FIII 9 -10的高阶多聚体才能完全促进通过整合素α5β1结合介导的细胞铺展。
Progress towards endometrial tissue engineering for modelling endometrial diseases and infertility is frustrated by the inability to mimic the fibronectin (FN) extracellular matrix required by human endometrial stromal cells (EnSCs). Here we show that this is because of the requirement to present integrin α5β1 (the FN receptor) ligands in specifically oriented, polyvalent displays; by engineering controlled self-assembly of the 9th–10th type III FN domain pair (FIII9–10, the minimal integrin α5β1 ligand) immobilised in a specific orientation to cell culture surfaces. The fraction of adherent EnSCs seen to spread increased significantly for the multimeric ligand surfaces in the order: tetramer > trimer > dimer > monomer. The extent of EnSC spread morphology also increased in the same order, with the tetrameric ligand supporting a morphology most similar to that supported by FN. Our data suggest that only higher-order multimers of FIII9–10 will fully promote cell spreading mediated through integrin α5β1 binding.