Production and separation of a trehalolipid biosurfactant
Production and separation of a trehalolipid biosurfactant
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DOI：
10.1016/j.bej.2018.07.006
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发表时间：
2018-11-15
影响因子：
3.9
通讯作者：
Martin, P. J.
中科院分区：
文献类型：
--
作者：
Bages-Estopa, S.;White, D. A.;Martin, P. J.
本文报道了海藻糖脂生物表面活性剂在发酵液中的发泡特性以及利用发泡实现产品回收的研究。海藻糖脂是由最近分离的海洋细菌红球菌产生的。 PML026，在摇瓶和生物反应器中。使用十六烷作为碳底物进行发酵。十六烷是一种消泡剂，可抑制发酵过程中泡沫的形成，这在生长和生产阶段具有有利的作用。这项工作的目的是提高海藻糖脂的生产并确定合适的培养基配方和工艺条件，以确保在发酵结束时耗尽十六烷底物，从而能够发生发泡，从而通过泡沫分馏实现产品分离。结果表明，在阈值生物表面活性剂浓度和残留量的十六烷的情况下，生物反应器中肉汤开始剧烈发泡。发现通过生物反应器冷凝器出口溢出的泡沫分离了总海卤脂的23-58%，产物富集度为2.3-1.4。这项研究首次证明了一种潜在的廉价且环保的策略，可以从乳化发酵液中分离海卤脂。 (C) 2018 Elsevier B.V. 保留所有权利。
This paper reports on a study of the foaming characteristics of trehalolipid biosurfactants in fermentation broths and the utilisation of foaming to enable product recovery. Trehalolipids were produced by a recently isolated marine bacterium, Rhodococcus sp. PML026, in shake flasks and bioreactors. Fermentations were conducted with hexadecane as the carbon substrate. Hexadecane is an antifoam agent which supresses foam formation during fermentation, an advantageous effect during the growth and production phases. The aim of this work was to improve trehalolipid production and identify a suitable media formulation and process conditions to ensure the hexadecane substrate was depleted by the end of the fermentation to enable foaming to occur, allowing for product separation by foam fractionation. The results show that at a threshold biosurfactant concentration and with a residual amount of hexadecane vigorous foaming of the broth commenced in the bioreactor. Foam overflowed through the bioreactor condenser exit was found to separate 23-58% of the total trehalolipids with a product enrichment of 2.3-1.4. This study demonstrates for the first time a potential inexpensive and environmentally friendly strategy to separate trehalolipids from an emulsified fermentation broth. (C) 2018 Elsevier B.V. All rights reserved.