Distribution of interstitial cells of Cajal in Meriones unguiculatus and alterations in the development of incomplete intestinal obstruction.
Distribution of interstitial cells of Cajal in Meriones unguiculatus and alterations in the development of incomplete intestinal obstruction.
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爪沙鼠 Cajal 间质细胞的分布及不完全性肠梗阻发生过程中的变化。
DOI：
10.14670/hh-28.1567
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发表时间：
2013-05
影响因子：
2
通讯作者：
Zhou, Deshan
中科院分区：
文献类型：
--
作者：
Wu, Bo;Liu, Li;Gao, Hengyu;Sun, Haimei;Xue, Hong;Li, Xiaoshuang;Zhang, Guoquan;Zhou, Deshan
Cajal间质细胞（interstitial cells of Cajal，ICCs）作为起搏细胞参与胃肠动力障碍，但其发病机制尚不清楚。蒙古沙鼠的胃肠道与人类的胃肠道具有相似的解剖学特征，但在这种动物的胃肠道中还没有ICC的研究报告。本研究首先观察了ICC在长爪沙鼠胃肠道的分布和形态特征。ICC主要分布于平滑肌层（ICC-IM）、肌间神经丛（ICC-MY）、小肠深肌丛（ICC-IM）和结肠环行肌层粘膜下层（ICC-SM）。从胃到结肠，ICC-IM的密度逐渐降低，而ICC-MY的密度逐渐增加。其次，我们比较了对照组和梗阻组之间ICC的差异，梗阻7天后ICC的数量没有显著差异。然而，在梗阻的第14天左右，数量减少。免疫反应性的模式也部分不同于对照组，即，经常观察到国际商会网络分散和中断。蛋白质印迹法显示，p-Kit和SCF显着减少，在扩张的肠道中的第14天。我们的研究结果表明，蒙古沙鼠可能是一个很好的动物模型，研究ICC的变化，可能有助于胃肠道动力障碍的发病机制。
The interstitial cells of Cajal (ICCs) act as pacemaker cells that are involved in gastrointestinal (GI) motility disorders, although the pathogenesis of these disorders is still unclear. The GI tract of Mongolian gerbils shares similar anatomical features with that of humans, but no investigation of ICCs has been reported in the GI tracts of this animal. In the present study, we first observed the distribution and morphological features of ICCs in the Mongolian gerbil GI tract. The ICCs were mainly distributed within the smooth muscle layers (ICC-IM), the myenteric plexus (ICC-MY), the deep muscular plexus in the small intestine (ICC-DMP) and the submucosal surface of the circular muscle layer in the colon (ICC-SM). The density of the ICC-IM gradually decreased from the stomach to the colon, whereas the density of the ICC-MY gradually increased. Second, we compared differences in the ICCs between the control and obstructed intestines, and no significant difference was observed in the number of ICCs after 7 days of obstruction. However, the numbers were reduced by approximately day 14 of obstruction. The pattern of immunoreactivity also partly differed from that of the control group, i.e., a scattered and interrupted network of ICCs was often observed. Western blotting revealed that p-Kit and SCF were significantly reduced in the dilated intestines by day 14. Our results indicate that the Mongolian gerbil may be a good animal model for studying changes in ICCs that may contribute to the pathogenesis of GI motility disorders.