Lipid class composition of membrane and raft fractions from brains of individuals with Alzheimer's disease
Lipid class composition of membrane and raft fractions from brains of individuals with Alzheimer's disease
复制标题
阿尔茨海默病患者大脑膜和筏部分的脂质类组成
DOI：
10.1016/j.bbrep.2019.100704
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发表时间：
2019
影响因子：
2.7
通讯作者：
Nishimura M.
中科院分区：
文献类型：
--
作者：
Kawatsuki A;Morita S-Y;Watanabe N;Hibino E;Mitsuishi Y;Sugi T;Murayama S;Nishimura M.
脑膜脂质稳态的扰动与阿尔茨海默病（AD）的发病机制有关。载脂蛋白E基因（APOE）的ε4等位基因以剂量依赖的方式增加了脑淀粉样蛋白-β积累和散发性AD发展的风险。apo基因型对脑脂质稳态的影响可能是与ε4等位基因相关的AD风险的基础。在本研究中，我们检测了apoeε 4对脑粗膜和筏子富集部分脂类组成的影响。我们采用基于酶促反应的方法定量测定磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酸和鞘磷脂。我们的研究结果表明，AD受试者的脑脂类组成既没有显著改变，也没有受到apoeε 4等位基因存在的影响。
Perturbation of the homeostasis of brain membrane lipids has been implicated in the pathomechanism of Alzheimer's disease (AD). The ε4 allele of the apolipoprotein E gene (APOE) confers an increased risk, in a dosage-dependent manner, for brain amyloid-β accumulation and the development of sporadic AD. An effect of theAPOEgenotype on brain lipid homeostasis may underlie the AD risk associated with the ε4 allele. In this research, we examined an effect ofAPOEε4 on the lipid class composition of crude membranes and raft-enriched fractions of brains. We applied enzymatic reaction-based methods for the quantification of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidic acid, and sphingomyelin. Our results indicate that brain lipid class composition was neither significantly altered in AD subjects nor affected by the presence of theAPOEε4 allele.