Prevalence of AIDS-associated retrovirus and antibodies among male homosexuals at risk for AIDS in Greenwich Village.
Prevalence of AIDS-associated retrovirus and antibodies among male homosexuals at risk for AIDS in Greenwich Village.
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格林威治村有艾滋病风险的男性同性恋者中艾滋病相关逆转录病毒和抗体的患病率。
DOI：
10.1089/aid.1.1983.1.407
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Volsky,DJ
中科院分区：
文献类型：
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作者：
Casareale,D;Dewhurst,S;Sonnabend,J;Sinangil,F;Purtilo,DT;Volsky,DJ
LAV/HTLV-III与获得性免疫缺陷综合征(AIDS)密切相关。我们对格林威治村几组男性同性恋者中艾滋病相关逆转录病毒和逆转录病毒抗体的流行率进行了研究并进行了关联。通过逆转录酶(RT)检测在培养的外周血淋巴细胞中检测到逆转录病毒，并通过病毒产生细胞系的建立和电子显微镜进行确认。在RT检测中，76%的艾滋病患者、93%的艾滋病相关复合体(ARC)患者、69%的全身性淋巴结病(LAS)患者和35%的无症状同性恋者的病毒呈阳性。在随机选择的11例病例中，有10例将病毒从RT阳性的淋巴细胞转移到CEM细胞系中。从8名异性恋者的淋巴细胞中未分离到病毒分离物。用我们建立的LAV/HTLV-III产生细胞系LAV-N1，用间接免疫荧光法检测血清中抗艾滋病相关病毒抗体，并用Western blotting进行验证。用蔗糖梯度超速离心法提纯LAV-N1病毒，用SDS-凝胶电泳法和免疫印迹法鉴定其蛋白图谱。LAV/HTLV-III的主要多肽为19、25-27、32、42和54kodalton。这些蛋白在与成人T细胞白血病病毒(ATLV)阳性血清的免疫印迹中不反应。因此，LAV-N1和ATLV在抗原性上没有相关性。在我们的LAV/HTLV-III抗体检测中，18例(100%)艾滋病患者，13例(100%)ARC患者，24例(69%)LAS患者和23例无症状同性恋者中9例(39%)血清阳性。异性对照均为阴性。经免疫印迹检测，所有IF阳性血清均含有针对特定病毒蛋白的抗体。71%的艾滋病患者、62%的艾滋病患者、38%的LAS和13%的无症状同性恋者的血清中检测到高滴度(≥1：1280)的LAV/HTLV-III型病毒抗体。我们的数据显示，LAV/HTLV-III抗体的存在与传染性疾病的存在相关
LAV/HTLV-III has been closely linked to the acquired immunodeficiency syndrome (AIDS). We have studied and correlated the prevalence of AIDS-associated retrovirus and retroviral antibodies in several groups of male homosexuals from Greenwich Village. Retrovirus was detected in cultured peripheral blood lymphocytes by testing for reverse transcriptase (RT) and confirmed by establishment of virus-producer cell lines, and electron microscopy. Seventy-six percent of patients with AIDS, 93% with AIDS-related complex (ARC), 69% with generalized lymphadenopathy (LAS), and 35% of asymptomatic homosexuals were positive for virus in the RT assay. Transmission of the virus from RT-positive lymphocytes into the CEM cell line was successful in 10 of 11 randomly chosen cases. No virus isolates were obtained from lymphocytes of 8 heterosexual individuals. Serum antibodies against AIDS-associated virus were detected by indirect immunofluorescence assay and confirmed by Western blotting, using an LAV/HTLV-III-producer cell line, LAV-N1, which we established. LAV-N1 virus was purified by ultracentrifugation through sucrose gradient and the pattern of its proteins was determined by SDS-gel electrophoresis and Western blotting using sera from an AIDS patient. The major polypeptides of LAV/HTLV-III (19, 25-27, 32, 42 and 54 kilodalton) were present. These proteins did not react in Western blots with sera positive for Adult T cell leukemia virus (ATLV). thus, LAV-N1 and ATLV were not antigenically related. In our assay for LAV/HTLV-III antibodies, 18 (100%) of patients with AIDS, 13 (100%) of patients with ARC, 24 (69%) of 35 patients with LAS and 9 (39%) of 23 asymptomatic homosexuals were sero-positive. Heterosexual controls were negative. All IF-positive sera tested by Western blot contained antibodies against specific viral proteins. High titers (≥ 1:1280) of serum antibodies against LAV/HTLV-III virus were detected in 71% of AIDS patients, 62% with ARC, 38% LAS and 13% among asymptomatic homosexuals. Our data show that the presence of LAV/HTLV-III antibodies correlates with the presence of infectious