Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat
Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat
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DOI：
10.1016/j.jplph.2007.05.001
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发表时间：
2007-10-01
影响因子：
4.3
通讯作者：
Esteves Vieira, Luiz Gonzaga
中科院分区：
文献类型：
--
作者：
Gruszka Vendruscolo, Eliane Cristina;Schuster, Ivan;Esteves Vieira, Luiz Gonzaga
亚热带地区。蛋白质的积累似乎是维持植物在逆境条件下生产力的一种很有前途的方法。然而，在携带由构成启动子控制的渗透保护剂编码基因的转基因植物中，观察到形态变化和生长减少。在胁迫诱导的启动子复合体AIPC的控制下，我们报道了水分亏缺对转Vigna aconitifolia Delta‘-pyrroline-5-cixylate synthetase(P5CS)的小麦植株的影响。P5CS编码Pro生物合成的关键调控酶。转基因小麦植株在缺水15天后表现出明显的反应。我们发现干旱是蛋白质积累的结果。转基因植株对水分亏缺的耐性主要是由于对氧化胁迫的保护机制，而不是由渗透调节引起的。(C)2007年爱思唯尔股份有限公司。版权所有。
subtropical regions. Accumulation of protine appears to be a promising approach to maintain the productivity of plants under stress condition. However, morphological alterations and growth reduction are observed in transgenic plants carrying genes coding for osmoprotectants controlled by constitutive promoters. We report here the effects of water deficit on wheat plants transformed with the Vigna aconitifolia Delta'-pyrroline-5-carboxylate synthetase (P5CS) cDNA that encodes the key regulatory enzyme in proline biosynthesis, under the control of a stress-induced promoter complex-AIPC. Transgenic wheat plants submitted to 15 days of water shortage presented a distinct response. We have found that drought resulted in the accumulation of protine. The tolerance to water deficit observed in transgenic plants was mainly due to protection mechanisms against oxidative stress and not caused by osmotic adjustment. (C) 2007 Elsevier GmbH. All rights reserved.