Prickle isoforms control the direction of tissue polarity by microtubule independent and dependent mechanisms
Prickle isoforms control the direction of tissue polarity by microtubule independent and dependent mechanisms
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DOI：
10.1242/bio.016162
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发表时间：
2016-03-15
期刊：
影响因子：
2.4
通讯作者：
Axelrod, Jeffrey D.
中科院分区：
文献类型：
--
作者：
Sharp, Katherine A.;Axelrod, Jeffrey D.
平面细胞极性信号传导指导许多上皮细胞平面内的细胞极化。虽然这些组织表现出一组核心模块蛋白质的不对称定位，但在果蝇中，不止一种机制将核心模块极化的方向与组织轴联系起来。一个信号系统建立了一个极性偏置的平行，顶端微管上的囊泡含有核心蛋白的交通。交换表达的差异表达的刺异构体，刺和刺腿，逆转的核心模块极化的方向。对近翅和前腹部的研究表明，这是由于它们对微管极性的不同控制。棘腿和棘腿也控制着远翅（D翼）和后腹（P腹）的极化方向。我们在这里报告，这发生在这些组织中不影响微管极性。因此，D翼的极性方向可能由一种独立于微管极性的新机制决定。在P-abd中，刺腿和棘腿通过微管极性独立机制解释至少两个方向线索。
Planar cell polarity signaling directs the polarization of cells within the plane of many epithelia. While these tissues exhibit asymmetric localization of a set of core module proteins, in Drosophila, more than one mechanism links the direction of core module polarization to the tissue axes. One signaling system establishes a polarity bias in the parallel, apical microtubules upon which vesicles containing core proteins traffic. Swapping expression of the differentially expressed Prickle isoforms, Prickle and Spiny-legs, reverses the direction of core module polarization. Studies in the proximal wing and the anterior abdomen indicated that this results from their differential control of microtubule polarity. Prickle and Spiny-legs also control the direction of polarization in the distal wing (D-wing) and the posterior abdomen (P-abd). We report here that this occurs without affecting microtubule polarity in these tissues. The direction of polarity in the D-wing is therefore likely determined by a novel mechanism independent of microtubule polarity. In the P-abd, Prickle and Spiny-legs interpret at least two directional cues through a microtubule-polarity-independent mechanism.