CARBON DIOXIDE REQUIREMENT OF VARIOUS SPECIES OF RUMEN BACTERIA
CARBON DIOXIDE REQUIREMENT OF VARIOUS SPECIES OF RUMEN BACTERIA
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DOI：
10.1128/jb.105.1.70-76.1971
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发表时间：
1971-01-01
影响因子：
3.2
通讯作者：
DEHORITY, BA
中科院分区：
文献类型：
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作者：
DEHORITY, BA
对11种32株瘤胃细菌的二氧化碳需要量进行了详细研究。将浓度不断升高的co2作为nahco3加入到特制的无co2培养基中，培养成管状，在氮气条件下接种。接种物中co2的前期消耗影响需要量；但是，似乎并没有涉及到媒介的复杂性和缓冲能力。8株反刍拟杆菌、3株琥珀酸拟杆菌、4株黄瘤球菌、2株多裂毛杆菌、1株溶淀粉琥珀酸单胞菌和2株溶纤维丁酸弧菌对co2有绝对需水量。在无co2培养基中，每个菌株一个b的生长响应不一致。溶纤维菌，白色瘤胃球菌和反刍硒单胞菌。另外6株b。随着CO2浓度的增加，溶纤维菌、反刍抗人乌杆菌和糊精溶剂琥珀酸弧菌的数量明显增加或受到刺激。elsdeniiB159是唯一一种似乎不需要绝对或部分二氧化碳的生物。对于那些以琥珀酸为主要最终产物的物种来说，其生长的开始和最佳生长都需要更高浓度的二氧化碳。
The carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. Increasing concentrations of CO2were added as NaHCO3to a specially prepared CO2-free medium which was tubed and inoculated under nitrogen. Prior depletion of CO2in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. An absolute requirement for CO2was observed for eight strains ofBacteroides ruminicola, three strains ofBacteroides succinogenes, four strains ofRuminococcus flavefaciens, two strains ofLachnospira multiparus, one strain ofSuccinimonas amylolytica, and two strains ofButyrivibrio fibrisolvens. Inconsistent growth responses were obtained in CO2-free media with one strain each ofB. fibrisolvens, Ruminococcus albus, andSelenomonas ruminantium. Growth of six additional strains ofB. fibrisolvens, and single strains ofEubacterium ruminantiumandSuccinivibrio dextrinosolvenswas markedly increased or stimulated by increasing concentrations of CO2.Peptostreptococcus elsdeniiB159 was the only organism tested which appeared to have no requirement, either absolute or partial, for CO2. Higher concentrations of CO2were required for the initiation of growth, as well as for optimal growth, by those species which produce succinic acid as one of their primary end products.