SUN proteins and nuclear envelope spacing.
SUN proteins and nuclear envelope spacing.
复制标题
DOI：
10.4161/19491034.2014.990857
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Starr DA
中科院分区：
文献类型：
--
作者：
Cain NE;Starr DA
核被膜由两层膜组成，两层膜相隔30-50 nm，但这两层膜如何均匀间隔一直是该领域的一个悬而未决的问题。核膜桥由内核膜SUN蛋白和外核膜KASH蛋白组成，被认为是设置和调节核膜间距的桥梁。我们直接测试了这一假设，通过检查核膜间距秀丽隐杆线虫动物缺乏EST-84，唯一的体细胞SUN蛋白。在大多数组织中，SUN/KASH桥不需要维持均匀的核膜间距。然而，在体壁肌核中，均匀间隔需要β-84。缩短300个氨基酸的<$A-84没有缩小核膜空间。虽然SUN蛋白可能在经历力的细胞中维持核膜间距方面发挥作用，但我们的数据表明它们在大多数细胞中是不稳定的。
The nuclear envelope consists of 2 membranes separated by 30–50 nm, but how the 2 membranes are evenly spaced has been an open question in the field. Nuclear envelope bridges composed of inner nuclear membrane SUN proteins and outer nuclear membrane KASH proteins have been proposed to set and regulate nuclear envelope spacing. We tested this hypothesis directly by examining nuclear envelope spacing in Caenorhabditis elegans animals lacking UNC-84, the sole somatic SUN protein. SUN/KASH bridges are not required to maintain even nuclear envelope spacing in most tissues. However, UNC-84 is required for even spacing in body wall muscle nuclei. Shortening UNC-84 by 300 amino acids did not narrow the nuclear envelope space. While SUN proteins may play a role in maintaining nuclear envelope spacing in cells experiencing forces, our data suggest they are dispensable in most cells.