ELECTROKINETIC CHARACTERIZATION OF EHRLICH ASCITES CARCINOMA CELL
ELECTROKINETIC CHARACTERIZATION OF EHRLICH ASCITES CARCINOMA CELL
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DOI：
10.1016/0014-4827(62)90309-9
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发表时间：
1962-01-01
影响因子：
3.7
通讯作者：
HEARD, DH
中科院分区：
文献类型：
--
作者：
COOK, GMW;SEAMAN, GVF;HEARD, DH
用胰蛋白酶、神经氨酸酶和低级醛处理标准盐水洗涤的埃利希腹水肿瘤细胞前后的pH-迁移率关系已被检查。用Warren法测定神经氨酸酶处理细胞的上清液中的唾液酸，用纸层析法测定胰蛋白酶处理细胞的上清液中的唾液酸。从分析数据和电动数据之间的相关性推断，该细胞的表面带有至少三个离子发生系统，唾液酸的羧基（pK约2.8）、未鉴定的酸性基团（pK约3.5 - 4.0）和碱性基团（pK约10）。
The pH-mobility relationships for standard saline washed Ehrlich ascites tumour cells before and after treatment with trypsin, neuraminidase and lower aldehydes have been examined. Supernatant fluids from neuraminidase-treated cells assayed for sialic acid by the method of Warren and supernatant from trypsin treated cells examined by paper chromatography. From a correlation between the analytical and electrokinetic data it is deduced that this cell''s surface bears at least three ionogenic systems, a carboxyl group (pK about 2.8), of a sialic acid, an unidentified acidic group(s) (pK about 3.5 - 4.0) and a basic group (pK about 10).