Genetic Diversity of O-Antigens in Hafnia alvei and the Development of a Suspension Array for Serotype Detection.
Genetic Diversity of O-Antigens in Hafnia alvei and the Development of a Suspension Array for Serotype Detection.
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蜂房哈夫尼亚 O 抗原的遗传多样性及用于血清型检测的悬浮芯片的开发
DOI：
10.1371/journal.pone.0155115
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Duan Z;Niedziela T;Lugowski C;Cao B;Wang T;Xu L;Yang B;Liu B;Wang L
肺泡Hafnia alvei是一种兼性棒状革兰氏阴性细菌，属于肠杆菌科。尽管自该属被确定以来已有50多年的历史，但人们对海夫尼亚种之间的变异知之甚少。o -抗原（o -多糖，OPS）的多样性被认为是细菌适应不同宿主和环境的主要因素。抗原变异也是致病力的一个重要因素，已被用于确定许多物种内的克隆。合成OPS所需的基因总是聚集在细菌染色体内。一种包括39种o型血清型的肺泡嗜血杆菌血清分型方案已被提出，但它与已知的OPS结构没有相关性，而且之前也没有报道描述OPS的遗传特征。在这项研究中，我们获得了21株不同脂多糖的肺泡H.菌株（根据先前免疫化学研究定义）的基因组序列。这是首次发现肺泡芽孢杆菌o抗原基因簇位于染色体mpo和gnd之间。所有的21个OPS基因簇均含有wzx基因和wzy基因，并表现出大量的多态性。我们开发了一种O血清型特异性的基于wz的悬浮阵列来检测我们在肺泡嗜血杆菌中发现的所有21种不同的OPS形式。据我们所知，这是第一份鉴定肺泡芽胞杆菌抗原变异的遗传特征和开发一种分子技术来鉴定和分类不同血清型的报告。
Hafnia alvei is a facultative and rod-shaped gram-negative bacterium that belongs to the Enterobacteriaceae family. Although it has been more than 50 years since the genus was identified, very little is known about variations among Hafnia species. Diversity in O-antigens (O-polysaccharide, OPS) is thought to be a major factor in bacterial adaptation to different hosts and situations and variability in the environment. Antigenic variation is also an important factor in pathogenicity that has been used to define clones within a number of species. The genes that are required to synthesize OPS are always clustered within the bacterial chromosome. A serotyping scheme including 39 O-serotypes has been proposed for H. alvei, but it has not been correlated with known OPS structures, and no previous report has described the genetic features of OPS. In this study, we obtained the genome sequences of 21 H. alvei strains (as defined by previous immunochemical studies) with different lipopolysaccharides. This is the first study to show that the O-antigen gene cluster in H. alvei is located between mpo and gnd in the chromosome. All 21 of the OPS gene clusters contain both the wzx gene and the wzy gene and display a large number of polymorphisms. We developed an O serotype-specific wzy-based suspension array to detect all 21 of the distinct OPS forms we identified in H. alvei. To the best of our knowledge, this is the first report to identify the genetic features of H. alvei antigenic variation and to develop a molecular technique to identify and classify different serotypes.