Can the Quality of Pearls from the Japanese Pearl Oyster (Pinctada fucata) be Explained by the Gene Expression Patterns of the Major Shell Matrix Proteins in the Pearl Sac?
Can the Quality of Pearls from the Japanese Pearl Oyster (Pinctada fucata) be Explained by the Gene Expression Patterns of the Major Shell Matrix Proteins in the Pearl Sac?
复制标题
DOI：
10.1007/s10126-010-9267-1
复制
发表时间：
2011-02-01
影响因子：
3
通讯作者：
Komaru, Akira
中科院分区：
文献类型：
--
作者：
Inoue, Nariaki;Ishibashi, Ryo;Komaru, Akira
对于珍珠养殖，珍珠贝被强行打开，并通过套膜移植物将细胞核植入性腺中。植入的外套膜移植物的外外套膜上皮细胞伸长，并在细胞核周围形成珍珠囊。珍珠囊分泌的贝壳基质蛋白在珍珠形成的调节中发挥着重要作用。最近，从珠母贝中鉴定出七种贝壳基质蛋白。然而，关于这些蛋白质的功能及其基因表达模式的信息很少。我们的研究旨在基于实时 PCR 分析，阐明珍珠类型、质量和珍珠囊中六种贝壳基质蛋白（msi60、n16、nacrein、msi31、prismalin-14 和 aspein）的基因表达模式之间的关系。培养约2个月后，从22个个体中收集了珍珠囊组织：12个优质（HP），9个低质量（LP），1个有机（ORG）珍珠形成。 12 颗 HP 珍珠的表面仅由珠光层组成；相比之下，九颗 LP 珍珠由珠光层和棱柱层组成。在所有个体中均检测到了六个目标基因的表达。然而，HP 个体中 msi31 的 Delta 阈值循环 (Delta C (T)) 显着高于 LP 个体（Mann-Whitney's U 检验，p = 0.02）。这意味着构成棱柱层框架的msi31的相对表达水平在LP个体中高于HP个体。
For pearl culture, the pearl oyster is forced open and a nucleus is implanted into the gonad with a mantle graft. The outer mantle epithelial cells of the implanted mantle graft elongate and surrounding the nucleus a pearl sac is formed. Shell matrix proteins secreted by the pearl sac play an important role in the regulation of pearl formation. Recently, seven shell matrix proteins were identified from the pearl oyster Pinctada fucata. However, there is a paucity of information on the function of these proteins and their gene expression patterns. Our study aims to elucidate the relationship between pearl type, quality, and gene expression patterns of six shell matrix proteins (msi60, n16, nacrein, msi31, prismalin-14, and aspein) in the pearl sac based on real-time PCR analysis. After culturing for about 2 months, the pearl sac tissues were collected from 22 individuals: 12 with high quality (HP), nine with low quality (LP), and one with organic (ORG) pearl formation. The surface of each of the 12 HP pearls was composed only of a nacreous layer; in contrast, that of the nine LP pearls was composed of nacreous and prismatic layers. The six target gene expressions were detected in all individuals. However, delta threshold cycle (Delta C (T)) for msi31 was significantly higher in the HP than in the LP individuals (Mann-Whitney's U test, p = 0.02). This means that the relative expression level of msi31, which constitutes the framework of the prismatic layer, was higher in the LP than in the HP individuals.