Usefulness of currently available methods for the diagnosis of Anisakis simplex allergy

Usefulness of currently available methods for the diagnosis of Anisakis simplex allergy
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DOI：
10.1034/j.1398-9995.2000.00471.x
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发表时间：
2000-07-01
期刊：
ALLERGY
影响因子：
12.4
通讯作者：
de Corres, LF
de Corres, LF
中科院分区：
医学1区
文献类型：
--
作者：
Lorenzo, S;Iglesias, R;de Corres, LF

文献摘要

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背景：异尖线虫病和异尖线虫过敏的血清学诊断很困难，因为许多异尖线虫抗原表现出交叉反应并发症。方法：用皮肤点刺试验、CAP-FEIA系统、Western blotting和与单抗UA3结合的O-脱糖化抗原的抗原捕获ELISA法，对无异尖过敏的受试者和确诊的异尖过敏受试者进行四种试验。结果：皮肤点刺试验、CAP-FEIA和抗原捕获ELISA法对异尖过敏血清的敏感性为100%，而Western blotting的敏感性为96%。抗原捕获酶联免疫吸附试验也有100%的特异性，而CAP-FEIA的特异性只有50%。在Western blotting中，检测到的条带都不是异尖过敏受试者或异尖过敏受试者所特有的。结论：根据这些结果，我们建议临床上怀疑异尖过敏的最合适的诊断方法是用UA3进行抗原捕获ELISA法，或者用UA3-EL ISA进行初步的皮肤点刺试验，结果为阳性。
Background: Serodiagnosis of anisakiosis and Anisakis allergy is difficult since many Anisakis antigens show cross-reactivity complications. In the present study, we assess the usefulness of the major immunologic methods currently available for the diagnosis of Anisakis allergy.Methods: Four tests (skin prick test, CAP-FEIA system, Western blotting, and an antigen-capture ELISA using O-deglycosylated antigen bound by the monoclonal antibody UA3) were applied to Anisakis-free subjects and subjects with confirmed Anisakis allergy.Results: The skin prick test, CAP-FEIA, and the antigen-capture ELISA identified Anisakis allergy sera with 100% sensitivity, while Western blotting showed 96% sensitivity. The antigen-capture ELISA also showed 100% specificity, but CAP-FEIA showed a specificity of only 50%. In Western blotting, none of the bands detected were specific for either Anisakis-free or Anisakis allergy subjects. The skin prick test was not applied to the Anisakis-free subjects, so its specificity could not be determined.Conclusions: On the basis of these results, we suggest that the most appropriate procedure for diagnosis of Anisakis allergy suspected on clinical grounds is the antigen-capture ELISA using UA3, or, alternatively, a preliminary skin prick test with a positive result subsequently confirmed by UA3-ELISA.