A horizontally acquired filamentous phage contributes to the pathogenicity of the plague bacillus
A horizontally acquired filamentous phage contributes to the pathogenicity of the plague bacillus
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DOI：
10.1111/j.1365-2958.2006.05570.x
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发表时间：
2007-02-01
影响因子：
3.6
通讯作者：
Carniel, Elisabeth
中科院分区：
文献类型：
--
作者：
Derbise, Anne;Chenal-Francisque, Viviane;Carniel, Elisabeth
鼠疫耶尔森氏菌，鼠疫杆菌，具有特殊的致病性，但其极端毒力的因素仍然是未知的。与其毒性较低的祖先假结核耶尔森氏菌的基因组比较，发现了一些Y。在它们的分歧之后获得的害虫特异性区域。其中之一可能编码前噬菌体（Ypf Phi），类似于与其他病原体中的毒力相关的丝状噬菌体。我们在这里表明，Ypf Phi形成丝状噬菌体颗粒感染其他Y。鼠疫分离物。虽然以前认为Ypf Phi仅限于东方分支，但我们的研究结果表明它是由Y.鼠疫的祖先在Antiqua和Medievalis菌株中，Ypf Phi基因组形成不稳定的附加体，而在Orientalis分离株中，它作为串联重复稳定整合。Ypf Phi基因组的缺失不影响Y。鼠疫菌在蚤前胃的定殖和封闭能力，但导致鼠疫菌的改变。鼠疫菌对小鼠的致病性结果表明，Y.鼠疫杆菌从典型的肠道病原体到高毒力鼠疫杆菌的过程中，伴随着获得不稳定的丝状噬菌体。尽管Ypf Phi在体外具有很高的不稳定性，但它的持续维持表明它赋予Y.在自然条件下，
Yersinia pestis, the plague bacillus, has an exceptional pathogenicity but the factors responsible for its extreme virulence are still unknown. A genome comparison with its less virulent ancestor Yersinia pseudotuberculosis identified a few Y. pestis-specific regions acquired after their divergence. One of them potentially encodes a prophage (Ypf Phi), similar to filamentous phages associated with virulence in other pathogens. We show here that Ypf Phi forms filamentous phage particles infectious for other Y. pestis isolates. Although it was previously suggested that Ypf Phi is restricted to the Orientalis branch, our results indicate that it was acquired by the Y. pestis ancestor. In Antiqua and Medievalis strains, Ypf Phi genome forms an unstable episome whereas in Orientalis isolates it is stably integrated as tandem repeats. Deletion of the Ypf Phi genome does not affect Y. pestis ability to colonize and block the flea proventriculus, but results in an alteration of Y. pestis pathogenicity in mice. Our results show that transformation of Y. pestis from a classical enteropathogen to the highly virulent plague bacillus was accompanied by the acquisition of an unstable filamentous phage. Continued maintenance of Ypf Phi despite its high in vitro instability suggests that it confers selective advantages to Y. pestis under natural conditions.