RE-UTILIZATION OF PYRIMIDINE NUCLEOTIDES DURING RAT-LIVER REGENERATION
RE-UTILIZATION OF PYRIMIDINE NUCLEOTIDES DURING RAT-LIVER REGENERATION
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DOI：
10.1042/bj2280027
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
DABEVA, MD
中科院分区：
文献类型：
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作者：
NIKOLOV, EN;DABEVA, MD
总酸溶性尿苷核苷酸池和尿苷和胞苷成分的总细胞和核RNA的特定放射性的变化进行了监测再生大鼠肝部分切除术后12天。证据是重新利用的嘧啶核苷酸来自细胞质RNA降解的新RNA的合成。循环的程度进行了评估，并更准确地确定了rRNA周转的真实速率。RNA中尿苷成分的再利用率在增殖期为7.0%/天，在增殖后期为3.2%/天，而胞苷核苷酸的再利用率更明显（分别为9.6%/天和18.1%/天）。结果表明，在大鼠肝细胞的细胞核和细胞质中存在部分区室化的嘧啶核苷三磷酸池。
The changes in the specific radioactivities of the pool of total acid-soluble uridine nucleotides and of uridine and cytidine components of total cellular and nuclear RNA were monitored in regenerating rat liver for 12 days after partial hepatectomy. Evidence is presented for the re-utilization of pyrimidine nucleotides derived from cytoplasmic RNA degradation for the synthesis of new RNA. The extent of recycling was assessed and the true rate of rRNA turnover determined more accurately. The reutilization of the uridine components of RNA was 7.0%/day during the proliferative and 3.2%/day during the post-proliferative phase, whereas that of the cytidine nucleotides was more pronounced (9.6%/day and 18.1%/day respectively). Results reveal the existence of partial compartmentalization of pyrimidine ribonucleoside triphosphate pools in the nucleus and cytoplasm of rat liver cells.