Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles.
Anaphase B spindle dynamics in Drosophila S2 cells: Comparison with embryo spindles.
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DOI：
10.1186/1747-1028-6-8
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发表时间：
2011-04-08
期刊：
影响因子：
2.3
通讯作者：
Scholey JM
中科院分区：
文献类型：
--
作者：
de Lartigue J;Brust-Mascher I;Scholey JM
在果蝇合胞囊胚期，胚胎后期B是由细胞周期开关启动的，其中微管负端解聚和向外滑动的极间微管正端空间重组的抑制触发纺锤体伸长。果蝇培养的S2细胞中的RNA干扰可能是鉴定该开关的新组分的有用工具，但鉴于纺锤体设计的多样性，首先确定两个系统中后期B机制的保护程度是重要的。在这些系统中，涉及极性通量和主轴伸长之间负相关的基本机制在质量上是相似的，但存在定量差异。在S2细胞中，极向通量仅受到部分抑制，B期后期纺锤体伸长率随抑制程度的增加而增加。此外，eb1标记的微管正端从两极向两极间微管重叠区重新分布，但这在S2细胞中比在胚胎中不那么明显。最后，与胚胎一样，微管蛋白FRAP实验显示，在靠近极点的区域进行光漂白后，恢复百分比减少。后期B开关的基本特征，包括抑制极向通量和生长的微管+端重组，在这些系统中是保守的。因此，S2细胞可能有助于快速识别这种开关的新成分。数量上的差异可能反映了胚胎纺锤体对快速流线型有丝分裂的适应性。
In the Drosophila melanogaster syncytial blastoderm stage embryo anaphase B is initiated by a cell cycle switch in which the suppression of microtubule minus end depolymerization and spatial reorganization of the plus ends of outwardly sliding interpolar microtubules triggers spindle elongation. RNA interference in Drosophila cultured S2 cells may present a useful tool for identifying novel components of this switch, but given the diversity of spindle design, it is important to first determine the extent of conservation of the mechanism of anaphase B in the two systems. The basic mechanism, involving an inverse correlation between poleward flux and spindle elongation is qualitatively similar in these systems, but quantitative differences exist. In S2 cells, poleward flux is only partially suppressed and the rate of anaphase B spindle elongation increases with the extent of suppression. Also, EB1-labelled microtubule plus ends redistribute away from the poles and towards the interpolar microtubule overlap zone, but this is less pronounced in S2 cells than in embryos. Finally, as in embryos, tubulin FRAP experiments revealed a reduction in the percentage recovery after photobleaching at regions proximal to the pole. The basic features of the anaphase B switch, involving the suppression of poleward flux and reorganization of growing microtubule plus ends, is conserved in these systems. Thus S2 cells may be useful for rapidly identifying novel components of this switch. The quantitative differences likely reflect the adaptation of embryonic spindles for rapid, streamlined mitoses.
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影响因子：
3.3
作者：
Kwon, M;Morales-Mulia, S;Scholey, JM
通讯作者：
Scholey, JM
影响因子：
7.8
作者：
Matos, Irina;Pereira, Antonio J.;Maiato, Helder
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影响因子：
9.2
作者：
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DOI：
10.1073/pnas.110149597
发表时间：
2000-06-06
影响因子：
11.1
作者：
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通讯作者：
Dixon, JE
影响因子：
2.9
作者：
Wang, Haifeng;Brust-Mascher, Ingrid;Scholey, Jonathan M.
通讯作者：
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