Dynamic Nuclear Polarization Nuclear Magnetic Resonance in Human Cells Using Fluorescent Polarizing Agents
Dynamic Nuclear Polarization Nuclear Magnetic Resonance in Human Cells Using Fluorescent Polarizing Agents
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DOI：
10.1021/acs.biochem.8b00257
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发表时间：
2018-08-07
期刊：
影响因子：
2.9
通讯作者：
Barnes, Alexander B.
中科院分区：
文献类型：
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作者：
Albert, Brice J.;Gao, Chukun;Barnes, Alexander B.
固态核磁共振（NMR）能够对复杂的非均匀样品中的分子结构和动力学进行原子分辨率表征，但它通常不敏感。动态核极化（DNP）可将NMR信号强度提高几个数量级，并可与魔角旋转（MAS）结合使用，以实现灵敏的高分辨率光谱分析。在这里，我们报告MAS DNP实验，第一次，在完整的人类细胞内，DNP增强>40倍，样品温度为
Solid state nuclear magnetic resonance (NMR) enables atomic-resolution characterization of the molecular structure and dynamics within complex heterogeneous samples, but it is typically insensitive. Dynamic nuclear polarization (DNP) increases the NMR signal intensity by orders of magnitude and can be performed in combination with magic angle spinning (MAS) for sensitive, high-resolution spectroscopy. Here we report MAS DNP experiments, for the first time, within intact human cells with >40-fold DNP enhancement and a sample temperature of