An experimental and clinical evaluation of lens transparency and aging.
An experimental and clinical evaluation of lens transparency and aging.
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晶状体透明度和老化的实验和临床评估。
DOI：
10.1093/geronj/38.3.293
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Lerman,S
中科院分区：
文献类型：
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作者：
Lerman,S
正常的人类晶状体是一个精确形成的结构，含有约65%的水和35%的有机物，后者主要是（>90%）结构蛋白。晶状体在 13 毫米胚胎阶段被封装，因此终生脱离直接血液供应。它为研究者提供了一个存在于类似于单细胞类型的组织培养系统的环境中并且可以在体外复制的器官。由于角膜和晶状体是透明的，因此可以直接监测体内形态和生化老化变化。最近的发展现在也使我们能够监测某些分子变化。因此，该器官可以作为研究体内衰老参数的独特模型。用于监测晶状体荧光的体内紫外裂隙灯光密度分析为我们提供了一种有用的诊断和预后筛查方法，以识别由于过度紫外线暴露（职业或意外）、光敏剂（例如补骨脂素）以及正常年龄相关的晶状体荧光增加而造成的晶状体光损伤。皮质混浊在老年人中也很常见。目前正在进行的体外核磁共振（人类和动物晶状体）和体内（动物实验）正在迅速阐明老化晶状体皮层中光散射区域发展的物理化学基础。本综述证明了利用生物物理方法，特别是光谱学和核磁共振分析来描绘和监测体内和体外眼晶状体中特定年龄相关参数的可行性。
The normal human lens is a precisely formed structure containing about 65% water and 35% organic matter, the latter being mainly (>90%) structural proteins. The lens becomes encapsulated at the 13 mm embryonic stage and is consequently divorced from a direct blood supply for life. It provides the investigator with an organ that exists in an environment analogous to a single-cell type of tissue culture system and can be reproduced in vitro. Since the cornea and lens are transparent one can directly monitor morphologic and biochemical aging changes in vivo. Recent developments now permit us to monitor certain molecular changes as well. Thus this organ can serve as a unique model for studying in vivo aging parameters. In vivo UV slit lamp densitography to monitor lens fluorescence provides us with a useful diagnostic and prognostic screening method to identify lens photodamage due to excess UV exposure (occupational or accidental), photosensitizers (e.g. Psoralens) as well as the normal age related increase in lenticular fluorescence. Cortical opacities are also a common finding in older individuals. In vitro NMR (human and animal lenses) and in vivo (animal experiments) currently in progress are rapidly elucidating the physicochemical basis for the development of light scattering areas in the aging lens cortex. This review demonstrates the feasibility of utilizing biophysical methods, in particular, optical spectroscopy and nmr analyses to delineate and monitor specific age related parameters in the ocular lens, in vivo as well as in vitro.