CORTICOTROPIN-RELEASING FACTOR RECEPTORS IN MOUSE SPLEEN - IDENTIFICATION OF RECEPTOR-BEARING CELLS AS RESIDENT MACROPHAGES
CORTICOTROPIN-RELEASING FACTOR RECEPTORS IN MOUSE SPLEEN - IDENTIFICATION OF RECEPTOR-BEARING CELLS AS RESIDENT MACROPHAGES
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DOI：
10.1210/endo-127-1-440
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发表时间：
1990-07-01
期刊：
影响因子：
4.8
通讯作者：
DESOUZA, EB
中科院分区：
文献类型：
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作者：
WEBSTER, EL;TRACEY, DE;DESOUZA, EB
促肾上腺皮质激素释放激素是机体对应激的神经内分泌、自主神经、行为和免疫反应的主要整合因子。本研究结合细胞分离和受体结合技术，对小鼠脾中表达促肾上腺皮质激素释放激素受体的细胞类型(S)进行了鉴定。放射自显影研究[125]酪氨酸度-绵羊CRF([125I]OCRF)结合部位在脾主要定位于红髓和边缘地带的CRF受体。[~(125)I]OCRF结合部位的分布模式与印度墨汁在同一切片中的吞噬细胞中聚集的模式非常相似。为了确定表达CRF受体的特定细胞类型(S)，根据其物理和功能特性对脾细胞群中的[125I]OCRF结合活性进行了评估。通过吞噬聚苯乙烯小球和用Monts-4膜标记巨噬细胞来鉴定每个组分中的巨噬细胞，Monts-4是一种针对常驻巨噬细胞的单抗。脾细胞通过附着在玻璃珠或Sephadex G-10柱上，吞噬羰基铁颗粒，以及不连续的Percoll梯度离心法进行分离。在所有分级方法中，[~(125)I]OCRF结合量与吞噬活性(r=0.75；P<；0.001)和Monts-4染色(r=0.84；P<；0.001)均显著相关，强烈提示CRF受体主要表达于常驻脾巨噬细胞上。然而，[125I]OCRF基本上没有与常驻或诱导的腹膜巨噬细胞或几种单核/巨噬细胞、B细胞或T细胞系特异性结合。虽然这些结果表明CRF受体的表达可能仅限于一群脾巨噬细胞，但它们不排除CRF受体可能由微环境中存在的因素诱导脾和其他免疫系统相关组织的常驻巨噬细胞上的可能性。
CRF is a primary integrator of the organism''s coordinated neuroendocrine, autonomic, behavioral, and immune responses to stress. In the present study the identity of the cell type(s) expressing CRF receptors in mouse spleen was determined using a combination of cell fractionation and receptor-binding techniques. Autoradiographic studies of the distribution of [125]Tyr.degree.-ovine CRF ([125I]oCRF)-binding sites in spleen localized CRF receptors primarily to the red pulp and marginal zones. The distribution pattern of [125I]oCRF-binding sites closely resembled the pattern of India ink accumulated in phagocytic cells in the same sections. To identify the specific cell type(s) expressing CRF receptors, [125I]oCRF-binding activity was evaluated in splenic cell populations fractionated on the basis of their physical and functional properties. Macrophages were identified in each fraction by their phagocytosis of polystyrene beads and membrane labeling with MONTS-4, a monoclonal antibody specific for resident macrophages. Spleen cells were fractionated by adherence to glass bead or Sephadex G-10 columns, phagocytosis of carbonyl iron particles, and centrifugation on discontinuous Percoll gradients. By all fractionation methods, there was a significant correlation of [125I]oCRF binding with both phagocytic activity (r = 0.75; P < 0.001) and MONTS-4 staining (r = 0.84; P < 0.001), strongly suggesting that CRF receptors are primarily expressed on resident splenic macrophages. However, there was essentially no specific binding of [125I]oCRF to either resident or elicited peritoneal macrophages or to several monocyte/macrophage, B-cell, or T-cell lines. While these results suggest that the expression of CRF receptors may be restricted to a population of splenic macrophages, they do not exclude the possibility that CRF receptors may be induced on resident macrophages in spleen and other immune system-related tissues by factors present in the microenvironment.