Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit
Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit
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从金樱子果肉中分离出的新型酸性多糖的结构表征和免疫调节活性
DOI：
10.1016/j.ijbiomac.2019.09.201
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发表时间：
2020-02-15
影响因子：
8.2
通讯作者：
Wu, Hui
中科院分区：
文献类型：
--
作者：
Zhan, Qiping;Wang, Qian;Wu, Hui
首次从蔷薇果果肉中分离到一种新的酸性多糖（PPRLMF-2），其M-w值为137,123 Da，具有三螺旋结构。结构表征表明，pprllf -2由鼠李糖（7.6%）、阿拉伯糖（26.5%）、木糖（3.5%）、甘露糖（0.9%）、葡萄糖（5.7%）、半乳糖（31.9%）和半乳糖醛酸（23.9%）组成。甲基化和核磁共振（1d和2D）分析表明，PPRLMF-2含有16种糖苷键。免疫调节活性实验表明，pprlpf -2能显著增强RAW 264.7细胞的吞噬能力、细胞因子的分泌和mRNA表达。此外，SR、GR、TLR-2和TLR-4是PPRLMF-2上调p-ERK、p-JNK、p-p38和p-p65的主要模式识别受体（PRRs）。这些结果表明，pprlpf -2可以通过激活MAPK5和nf - κ B信号通路来识别巨噬细胞的PRRs，从而增强其免疫调节活性。该研究为PPRLMF-2作为一种有吸引力的免疫调节功能食品提供了重要的启示。(C) 2019 Elsevier B.V.版权所有
A novel acid polysaccharide (PPRLMF-2) with the M-w of 137,123 Da and a triple-helix conformation was first isolated from the pulp of Rosa laevigata Michx fruit. Structural characterization showed that PPRLMF-2 consisted of rhamnose (7.6%), arabinose (26.5%), xylose (3.5%), mannose (0.9%), glucose (5.7%), galactose (31.9%) and galacturonic acid (23.9%). The methylation and NMR (1 D and 2D) analysis revealed that PPRLMF-2 contained 16 types of glycosidic linkages. The immunomodulatory activity assays indicated that PPRLMF-2 could significantly enhance phagocytosis, the secretion and mRNA expression of cytokines in RAW 264.7 cells. Additionally, SR, GR, TLR-2, and TLR-4 were the main pattern recognition receptors (PRRs) of PPRLMF-2 to upregulate the p-ERK, p-JNK, p-p38, and p-p65. These results suggested that PPRLMF-2 could recognize the PRRs of the macrophages to enhance the immunomodulatory activity via activation of the MAPK5 and NF-kappa B signaling pathways. This study provides important implications of PPRLMF-2 as an attractive immunomodulatory functional food. (C) 2019 Elsevier B.V. All rights reserved.