High-content analysis of cancer genome DNA alterations.
High-content analysis of cancer genome DNA alterations.
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癌症基因组 DNA 改变的高内涵分析。
DOI：
10.1016/j.gde.2008.01.005
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发表时间：
2008
影响因子：
4
通讯作者：
Powers,Scott
中科院分区：
文献类型：
--
作者：
Degenhardt,YanY;Wooster,Richard;McCombie,RichardW;Lucito,Robert;Powers,Scott
新技术以及协调一致的蛮力方法增加了可以表征基因组DNA改变的内容（基因数量）。最近的进展包括检测多种癌症类型中关键激酶基因（BRAF，EGFR和PIK 3CA）的激活点突变：初步了解癌症中可能突变的整个基因库;通过DNA拷贝数改变的高分辨率分析发现新的致癌基因;以及生物信息学驱动的致癌基因融合发现。高含量的启动子甲基化检测系统已被用于发现额外的甲基化基因，并为某些肿瘤的干细胞起源提供了证据。其中一些进展对新疗法的开发和临床试验产生了重大影响。
New technologies as well as concerted brute-force approaches have increased the content (number of genes) that can be characterized for genomic DNA alterations. Recent advances include the detection of activating point mutations in key kinase genes (BRAF, EGFR, and PIK3CA) in multiple cancer types: preliminary insight into the entire repertoire of genes that can be mutated in cancer; the discovery of new oncogenes by high-resolution profiling of DNA copy number alterations; and the bioinformatic-driven discovery of oncogenic gene fusions. High-content promoter methylation detection systems have been used to discover additional methylated genes and have provided evidence for a stem cell origin for certain tumors. Some of these advances have had significant impact on the development and clinical testing of new therapeutics.