Proteomic atlas of the human olfactory bulb
Proteomic atlas of the human olfactory bulb
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DOI：
10.1016/j.jprot.2012.05.011
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发表时间：
2012-07-16
影响因子：
3.3
通讯作者：
Santamaria, Enrique
中科院分区：
文献类型：
--
作者：
Fernandez-Irigoyen, Joaquin;Corrales, Fernando J.;Santamaria, Enrique
嗅球（OB）是大脑中处理嗅觉信息的第一个部位，其失调与神经退行性疾病有关。虽然不同的努力已经作出了深入的表征人脑蛋白质组，人类OB的蛋白质组成仍然在很大程度上未被探索。我们已经进行了全面的分析，人类OB蛋白质组采用蛋白质和肽的分馏方法，然后通过LC-MS/MS，确定1529种蛋白质，对应于1466个独特的蛋白质，这代表了7倍的增加，相对于以前的OB蛋白质组描述翻译模型的蛋白质组覆盖。生物信息学分析表明，OB的蛋白质组分参与了大量的生物过程，突出了水解酶和磷酸酶活性以及核苷酸和RNA结合活性。有趣的是，631个OB蛋白在大规模人脑蛋白质组计划（HBPP）研究的蛋白质数据集中没有描述。特别是，这些差异蛋白的一个子集主要涉及轴突导向，阿片类信号，神经递质受体结合，和突触可塑性。两者合计，这些结果增加了我们对人类OB的分子组成的知识，并可能有助于了解嗅觉系统的分子基础及其疾病的病因。(c)2012爱思唯尔有限公司版权所有。
The olfactory bulb (OB) is the first site for the processing of olfactory information in the brain and its deregulation is associated with neurodegenerative disorders. Although different efforts have been made to characterize the human brain proteome in depth, the protein composition of the human OB remains largely unexplored. We have performed a comprehensive analysis of the human OB proteome employing protein and peptide fractionation methods followed by LC-MS/MS, identifying 1529 protein species, corresponding to 1466 unique proteins, which represents a 7-fold increase in proteome coverage with respect to previous OB proteome descriptions from translational models. Bioinformatic analyses revealed that protein components of the OB participated in a plethora of biological process highlighting hydrolase and phosphatase activities and nucleotide and RNA binding activities. Interestingly, 631 OB proteins identified were not previously described in protein datasets derived from large-scale Human Brain Proteome Project (HBPP) studies. In particular, a subset of these differential proteins was mainly involved in axon guidance, opioid signaling, neurotransmitter receptor binding, and synaptic plasticity. Taken together, these results increase our knowledge about the molecular composition of the human OB and may be useful to understand the molecular basis of the olfactory system and the etiology of its disorders. (c) 2012 Elsevier B.V. All rights reserved.