Effect of mechanical compression on the lumbar nerve root: Localization and changes of intraradicular inflammatory cytokines, nitric oxide, and cyclooxygenase
Effect of mechanical compression on the lumbar nerve root: Localization and changes of intraradicular inflammatory cytokines, nitric oxide, and cyclooxygenase
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DOI：
10.1097/01.brs.0000171910.97937.0e
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发表时间：
2005-08-01
期刊：
影响因子：
3
通讯作者：
Yoshizawa, H
中科院分区：
文献类型：
--
作者：
Kobayashi, S;Baba, H;Yoshizawa, H
研究设计。目的：探讨机械压迫引起的神经根内炎症。为了探讨神经根疼痛的机制，本研究采用腰神经根压迫模型。背景资料摘要。炎症部位疼痛的表现与巨噬细胞释放介质密切相关。然而，由于机械压迫导致神经根炎症的介质几乎仍然未知。本研究用夹子压迫犬第7腰椎神经根3周，观察压迫引起的变化。采用亲和素-生物素-过氧化物酶复合物法进行免疫组化，观察压迫后T细胞（CD45）和巨噬细胞（Mac-1）的变化。采用抗白细胞介素-1 （IL-1）、肿瘤坏死因子- α (tnf - α)、诱导型一氧化氮合酶（i-NOS）和环氧化酶(COX)-1和2的抗体检测神经根受压引起的这些介质的定位和变化。在对照动物中，检测到驻留T细胞，但未检测到巨噬细胞。IL-1 β和COX-2在雪旺细胞和血管内皮细胞中呈阳性，COX-1在血管内皮细胞中呈阳性。然而，没有细胞显示tnf - α或i-NOS阳性。神经根受压后，脱髓鞘神经纤维中出现大量T细胞和巨噬细胞。巨噬细胞IL-1 β、tnf - α、i-NOS、cox -2阳性。炎性细胞因子、NO和COX-2可能深度参与机械压迫引起的根性炎，这些介质似乎在根痛的表现中起重要作用。
Study Design. Investigation of intraradicular inflammation induced by mechanical compression.Objective. To investigate the mechanism of nerve root pain, this study used a lumbar nerve root compression model.Summary of Background Data. The manifestation of pain at sites of inflammation has a close relationship with the release of mediators from macrophages. However, the mediators involved in inflammation of nerve roots as a result of mechanical compression remain almost unknown.Methods. In this study, the seventh lumbar nerve root of dogs was compressed with a clip for 3 weeks to observe the changes caused by compression. Immunohistochemistry was performed using the avidin-biotin-peroxidase complex method to observe the changes of T cells (CD45) and macrophages (Mac-1) after compression. Antibodies against as interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), inducible nitric oxide synthase (i-NOS), and cyclooxygenase (COX)-1 and 2 were used to examine the localization and changes of these mediators caused by nerve root compression.Results. In control animals, resident T cells were detected, but there were no macrophages. IL-1 beta and COX-2 were positive in the Schwann cells and vascular endothelial cells, while COX-1 was detected in the vascular endothelial cells. However, no cells showed TNF-alpha or i-NOS positively. After nerve root compression, numerous T cells and macrophages appeared among the demyelinized nerve fibers. The macrophages were positive for IL-1 beta, TNF-alpha, i-NOS, and COX-2.Conclusion. Inflammatory cytokines, NO, and COX-2 may be deeply involved in radiculitis caused by mechanical compression, and these mediators seem to be important in the manifestation of root pain.