Flow‐type cyanide sensor using an immobilized microorganism
Flow‐type cyanide sensor using an immobilized microorganism
复制标题
使用固定化微生物的流动式氰化物传感器
DOI：
10.1002/elan.1140081005
复制
发表时间：
1996
期刊：
影响因子：
3
通讯作者：
I. Karube
中科院分区：
文献类型：
--
作者：
K. Ikebukuro;M. Honda;K. Nakanishi;Y. Nomura;Y. Masuda;K. Yokoyama;Y. Yamauchi;I. Karube
描述了一种利用酿酒酵母检测氰化物的流动系统。该传感器系统由两个氧电极和固定化酿酒酵母组成，其呼吸活性由氧电极测量。因为氰化物抑制酿酒酵母的呼吸，所以可以通过测量这种抑制来检测它。构建了两种类型的系统，并对其进行了表征和比较。一种是将酿酒酵母固定在纤维素膜上的膜式流动系统。另一种是反应器式流动系统，将酿酒酵母固定在玻璃微珠上。传感器的响应范围为0~15μM。反应堆式传感器系统具有较低的检测下限(0.15μM)和较好的稳定性(16天)。
A flow system for cyanide detection using Saccharomyces cerevisiae is described. This sensor system consists of two oxygen electrodes and immobilized S. cerevisiae whose respiration activity is measured by the oxygen electrodes. Since cyanide inhibits S. cerevisiae's respiration, it can be detected by measuring this inhibition. Two types of systems were constructed, characterized and compared. One is a membrane-type flow system in which S. cerevisiae was immobilized onto a cellulose membrane. The other is a reactor-type flow system in which S. cerevisiae was immobilized onto the glass beads. The sensor response was examined in the range from 0 to 15μM. The reactor-type sensor system displayed lower detection limit (0.15 μM) and better stability (16 days).