Localization of osteopontin in oviduct tissue and eggshell during different stages of the avian egg laying cycle
Localization of osteopontin in oviduct tissue and eggshell during different stages of the avian egg laying cycle
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DOI：
10.1016/j.jsb.2003.08.007
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发表时间：
2003-09-01
影响因子：
3
通讯作者：
Arias, JL
中科院分区：
文献类型：
--
作者：
Fernandez, MS;Escobar, C;Arias, JL
禽蛋壳是一种无细胞生物陶瓷，含有有机和无机相，在鸡蛋沿输卵管移动的过程中依次组装。正如在其他矿化组织中所证明的那样，蛋壳的矿化受到细胞外基质蛋白的调节，特别是蛋白多糖的阴离子侧链。其中，在禽类的蛋壳和输卵管中发现了骨桥蛋白。然而，它在蛋壳形成过程中在蛋壳或不同输卵管区域的精确定位，以及它的功能还没有被确定。用抗骨桥蛋白抗体(OPN-1)研究了OPN-1在输卵管峡部、红色峡部和壳腺中的免疫定位，以及在卵壳中的免疫定位。在蛋壳中，骨桥蛋白定位于未矿化的壳膜纤维的核心、乳房底部和栅栏的最外层。在输卵管中，OPN 1定位于纤毛上皮，而不定位于峡部的管状腺细胞、红色峡部的纤毛上皮细胞和壳腺的非纤毛上皮细胞。骨桥蛋白在每个输卵管区域的出现，与卵子在该区域的伴随存在相吻合。考虑到已报道的骨桥蛋白在其他矿化系统中的抑制作用，以及它主要存在于蛋壳的非矿化部分和最外层，表明该分子可能是调节蛋壳钙化的机制的一部分。(C)2003 Elsevier Inc.保留所有权利。
The avian eggshell is an acellular bioceramic containing organic and inorganic phases that are sequentially assembled during the time the egg moves along the oviduct. As it has been demonstrated in other mineralized tissues, mineralization of the eggshell is regulated by extracellular matrix proteins especially the anionic side chains of proteoglycans. Among them, osteopontin has been found in the avian eggshell and oviduct. However, its precise localization in the eggshell or in different oviduct regions during eggshell formation, nor its function have been established. By using anti-osteopontin antibody (OPN 1), we studied its immunolocalization in the isthmus, red isthmus and shell gland of the oviduct, and in the eggshell during formation. In the eggshell, osteopontin was localized in the core of the non-mineralized shell membrane fibers, in the base of the mammillae and in the outermost part of the palisade. In the oviduct, OPN 1 was localized in the ciliated epithelial but not in the tubular gland cells of the isthmus, in the ciliated epithelial cells of the red isthmus, and in the non-ciliated epithelial cells of the shell gland. The occurrence of osteopontin in each of the oviduct regions, coincided with the concomitant presence of the egg in such region. Considering the reported inhibitory function of osteopontin in other mineralized systems, together with its main occurrence in the non-mineralized parts of the eggshell and at the outermost part of the shell, suggests that this molecule could be part of the mechanism regulating the eggshell calcification. (C) 2003 Elsevier Inc. All rights reserved.