Parathyroid hormone inhibitors: comparison of biological activity in bone- and skin-derived tissue.
Parathyroid hormone inhibitors: comparison of biological activity in bone- and skin-derived tissue.
复制标题
甲状旁腺激素抑制剂：骨源性组织和皮肤源性组织中生物活性的比较。
DOI：
--
复制
发表时间：
1979
影响因子：
5.8
通讯作者：
S. Krane
中科院分区：
文献类型：
--
作者：
S. Goldring;J. Mahaffey;M. Rosenblatt;J. Dayer;J. Potts;S. Krane
摘要为了评价甲状旁腺激素（PTH）和两种合成的PTH抑制剂的生物学特性，利用来自两种人体组织的单层细胞培养物。一个细胞培养系统由人骨巨细胞瘤制备，另一个由人新生儿包皮制备。当细胞培养物与PTH孵育时，从这两种组织获得的细胞中cAMP水平增加。PTH的表观亲和力常数（在真皮和肿瘤衍生细胞中分别为1.2 × 108和1.6 × 108 M）以及在两种人细胞培养物中对PTH诱导的cAMP含量增加产生50%抑制的合成PTH类似物浓度（类似物[Nle-8，Nle-18，Tyr-34]牛PTH-（3-34）酰胺在真皮和肿瘤衍生细胞中分别为5 × 108和8 × 108 M）与先前采用犬肾组织制备的膜的研究中观察到的值几乎相同。虽然不被认为是PTH的靶组织，但来自人的成纤维细胞...
ABSTRACT To evaluate the biological properties of parathyroid hormone (PTH) and two synthetic inhibitors of PTH, monolayer cell cultures derived from two human tissues were utilized. One cell culture system was prepared from human giant cell tumors of bone and the other from human neonatal foreskin. The level of cAMP was increased in cells obtained from both of these tissues when the cell cultures were incubated with PTH. The apparent affinity constants for PTH (1.2 × 108 and 1.6 × 108 M in dermal and tumor-derived cells, respectively) and the concentrations of the synthetic PTH analogs which produced 50% inhibition of PTH-induced increases in cAMP content in the two human cell cultures (5 × 108 and 8 × 108 M for the analog [Nle-8, Nle-18, Tyr-34]bovine PTH-(3-34) amide in dermal and tumor-derived cells, respectively) were nearly identical to the values previously observed in studies employing membranes prepared from canine renal tissue. Although not regarded as a PTH target tissue, fibroblasts from human...