EFFECTS OF ANTIBODIES, TRYPSIN, AND TRYPSIN-INHIBITORS ON SUSCEPTIBILITY OF NEONATES TO ROTAVIRUS INFECTION
EFFECTS OF ANTIBODIES, TRYPSIN, AND TRYPSIN-INHIBITORS ON SUSCEPTIBILITY OF NEONATES TO ROTAVIRUS INFECTION
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DOI：
10.1128/jcm.13.1.22-29.1981
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发表时间：
1981-01-01
影响因子：
9.4
通讯作者：
HOLMES, IH
中科院分区：
文献类型：
--
作者：
MCLEAN, BS;HOLMES, IH
抗轮状病毒分泌型伊加的水平进行了测定，酶联免疫吸附试验在初乳和牛奶样本，每天收集的前5天产后从49个母亲母乳喂养他们的婴儿。这些乳汁分泌物样品的胰蛋白酶抑制能力通过它们抑制α-胰蛋白酶水解的能力来评估。胰蛋白酶法制备N-苯甲酰基-DL-精氨酸-对硝基苯胺。将这些母乳喂养婴儿和另外43名奶瓶喂养婴儿的粪便按个体合并，并通过EM检查轮状病毒。采用明胶水解试验估计粪便胰蛋白酶水平。母乳喂养的婴儿感染轮状病毒的可能性明显低于奶瓶喂养的婴儿，粪便胰蛋白酶活性也明显低于奶瓶喂养的婴儿。母乳喂养的婴儿谁没有排泄轮状病毒超过5天的时间内收到显着更高的抗轮状病毒分泌型伊加的胰蛋白酶抑制能力的牛奶或两者都比母乳喂养的婴儿谁感染轮状病毒。1例可能的母亲轮状病毒感染在怀孕期间，产生了很大的升高，持续2年的乳抗轮状病毒分泌伊加水平，被检测到。人乳中的抗体和胰蛋白酶抑制剂都可以保护新生儿在出生后的前5天免受轮状病毒感染。
Levels of antirotaviral secretory IgA were measured by enzyme-linked immunosorbent assay in colostrum and milk samples collected daily for the first 5 days postpartum from 49 mothers breast-feeding their infants. The trypsin-inhibitory capacity of these lacteal secretion samples was assessed by their ability to inhibit the hydrolysis of .alpha.-N-benzoyl-DL-arginine-p-nitroanilide by trypsin. Stools passed by these breast-fed infants and by an additional 43 bottle-fed infants were pooled by individual and examined by EM for rotavirus. Stool trypsin levels were estimated with the gelatin hydrolysis test. Breast-fed infants were significantly less likely to become infected with rotavirus and showed significantly lower stool tryptic activity than did bottle-fed infants. Breast-fed infants who did not excrete rotavirus over the 5 day period received milk of significantly higher antirotaviral secretory IgA of trypsin-inhibitory capacity or both than breast-fed infants who were infected with rotavirus. A case of probable maternal rotavirus infection during pregnancy, producing greatly elevated lacteal antirotaviral secretory IgA levels lasting for 2 yr, was detected. Both antibodies and trypsin inhibitors in human milk can be associated with the protection of neonates against rotavirus infection in the first 5 days of life.