LXYL-P1-2 immobilized on magnetic nanoparticles and its potential application in paclitaxel production
LXYL-P1-2 immobilized on magnetic nanoparticles and its potential application in paclitaxel production
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磁性纳米粒子固定化LXYL-P1-2及其在紫杉醇生产中的潜在应用
DOI：
10.1016/j.ejbt.2020.12.005
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发表时间：
2021-03
影响因子：
2.7
通讯作者：
Ping Zhu
中科院分区：
文献类型：
--
作者：
Zou Sen;Tian-Jiao Chen;Li Danyang;Fan Shuai;Zhaoyong Yang;Ping Zhu
摘要背景LXYL-P1 -2是第一个被报道的糖苷水解酶，它能催化7-β-木糖基-10-脱乙酰紫杉醇（XDT）转化为10-脱乙酰紫杉醇（DT）。以XDT为前体，将LXYL-P1-2与10-脱乙酰基巴卡亭III-10-β-O-乙酰转移酶（DBAT）一锅法合成紫杉醇，使LXYL-P1-2成为一种非常有前途的紫杉醇工业化生产酶。本研究的目的是研究LXYL-P1-2稳定在磁性纳米颗粒上的催化潜力，其表面由Ni 2+固定的交联Fe3O4@Histidine修饰。结果基质粒径为20- 40 nm。固定化LXYL-P1-2催化XDT的Km值（0.145mM）低于游离酶的Km值（0.452mM），固定化酶的kcat/Km值（12.952mMs-1）高于游离形式（8.622mMs-1）。固定化形式保持50%的原始活性后，15个循环的重复使用。固定化LXYL-P1-2在4°C保存30 d后，其稳定性较游离态有明显提高，保持了84.67%的初始活性。结论本研究不仅为生物催化合成DT提供了一种有效的方法，而且为磁性材料固定化技术的应用提供了新的思路。如何引用邹S，陈天健，李迪扬，等.磁性纳米粒子固定化LXYL-P1-2及其在紫杉醇生产中的潜在应用。50.第50章：一个女人https://doi.org/10.1016/j.ejbt.2020.12.005
Abstract Background LXYL-P1-2 is the first reported glycoside hydrolase that can catalyze the transformation of 7-β-xylosyl-10-deacetyltaxol (XDT) to 10-deacetyltaxol (DT) by removing the d -xylosyl group at the C-7 position. Successful synthesis of paclitaxel by one-pot method combining the LXYL-P1-2 and 10-deacetylbaccatin III-10-β-O-acetyltransferase (DBAT) using XDT as a precursor, making LXYL-P1-2 a highly promising enzyme for the industrial production of paclitaxel. The aim of this study was to investigate the catalytic potential of LXYL-P1-2 stabilized on magnetic nanoparticles, the surface of which was modified by Ni2+-immobilized cross-linked Fe3O4@Histidine. Results The diameter of matrix was 20–40nm. The Km value of the immobilized LXYL-P1-2 catalyzing XDT (0.145mM) was lower than that of the free enzyme (0.452mM), and the kcat/Km value of immobilized enzyme (12.952mMs−1) was higher than the free form (8.622mMs−1). The immobilized form maintained 50% of its original activity after 15 cycles of reuse. In addition, the stability of immobilized LXYL-P1-2, maintained 84.67% of its initial activity, improved in comparison with free form after 30d storage at 4°C. Conclusions This investigation not only provides an effective procedure for biocatalytic production of DT, but also gives an insight into the application of magnetic material immobilization technology. How to cite Zou S, Chen TJ, Li DY, et al. LXYL-P1-2 immobilized on magnetic nanoparticles and its potential application in paclitaxel production. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.005
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DOI：
10.3390/biotech10010001
发表时间：
2020-12-28
期刊：
Biotech (Basel (Switzerland))
影响因子：
--
作者：
通讯作者：
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通过定向进化提高糖苷水解酶 LXYL-P1-2 的催化性能。
DOI：
10.3390/molecules22122133
发表时间：
2017-12-04
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Chen JJ;Liang X;Li HX;Chen TJ;Zhu P
通讯作者：
Zhu P
影响因子：
9.5
作者：
Y. Zhang;D. Li;Meng Yu;Wanfu Ma;Jia Guo;Changchun Wang
通讯作者：
Y. Zhang;D. Li;Meng Yu;Wanfu Ma;Jia Guo;Changchun Wang
影响因子：
3.8
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Han Zhang;Shao‐Feng Hua;Cong-qiang Li;Lei Zhang;Yunchang Fan;P. Duan
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Han Zhang;Shao‐Feng Hua;Cong-qiang Li;Lei Zhang;Yunchang Fan;P. Duan
DOI：
10.1016/j.ijbiomac.2020.06.227
发表时间：
2020-11-01
影响因子：
8.2
作者：
Govarthanan, M.;Jeon, Chang-Hyun;Kim, Woong
通讯作者：
Kim, Woong