AN IMMUNOCYTOCHEMICAL AND BIOCHEMICAL-STUDY OF THE MICROTUBULE-ASSOCIATED PROTEIN-TAU DURING POSTLESION AFFERENT REORGANIZATION IN THE HIPPOCAMPUS OF ADULT-RATS
AN IMMUNOCYTOCHEMICAL AND BIOCHEMICAL-STUDY OF THE MICROTUBULE-ASSOCIATED PROTEIN-TAU DURING POSTLESION AFFERENT REORGANIZATION IN THE HIPPOCAMPUS OF ADULT-RATS
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DOI：
10.1016/0006-8993(87)90590-7
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发表时间：
1987-09-01
期刊：
影响因子：
2.9
通讯作者：
CACERES, A
中科院分区：
文献类型：
--
作者：
BUSCIGLIO, J;FERREIRA, A;CACERES, A
微管相关蛋白（MAP）Tau的单克隆抗体被用来检查这个分子的命运在损伤后传入重组在成年大鼠海马。免疫细胞化学分析进行后，内嗅皮层（EC）的单方面破坏的齿状回。在非失神经海马，Tau免疫反应性检测平行轴突和苔藓纤维，没有染色存在于神经元细胞体和树突。EC损伤后2d，同侧齿状回分子层（ML）外2/3的Tau免疫反应性显著降低，而同一ML内1/3的Tau免疫反应性显著增加。随后是一个非常快速的恢复Tau免疫反应性的外2/3的失神经ML，这是由10天后病变几乎是相同的对侧非失神经ML。类似的现象，观察到在其他地区的海马神经支配的EC病变。Tau免疫反应性在失神经海马的修改也伴随着这种异质组的MAP的多肽组成的变化，所揭示的免疫印迹分析在不同的损伤后间隔获得的海马提取物，这些变化涉及的低分子量迁移Tau免疫反应性多肽的快速和显着增加。目前的观察结果表明，重要的修饰Tau蛋白发生在去传入海马，一种现象，这可能与损伤后轴突生长过程中的微管聚合的调节。
A monoclonal antibody against the microtubule-associated protein (MAP) Tau was used to examine the fate of this molecule during post-lesion afferent reorganization in the hippocampus of adult rats. An immunocytochemical analysis was carried out in the dentate gyrus after unilateral destruction of the entorhinal cortex (EC). In the non-denervated hippocampus, Tau immunoreactivity was detected in parallel axons and mossy fibers; no staining was present in neuronal cell bodies and dendrites. A significant decrease in Tau immunoreactivity was detected in the outer 2/3 of the ipsilateral dentate gyrus molecular layer (ML) 2 days after and EC lesion, whereas staining in the inner 1/3 of the same ML increased considerably. This was followed by a very rapid recovery of Tau immunoreactivity in the outer 2/3 of the denervated ML, which by 10 days post-lesion was almost identical to that of the contralateral non-denervated ML. A similar phenomenon was observed in other regions of the hippocampus denervated by the EC lesion. The modifications in Tau immunoreactivity in the denervated hippocampus were also accompanied by changes in the polypeptide composition of this heterogeneous group of MAPs, as revealed by immunoblot analysis of hippocampal extracts obtained at different post-lesion interval; these changes involved a rapid and significant increase in low molecular weight migrating Tau-immunoreactive polypeptides. The present observations indicate that important modifications in Tau protein occur in the deafferented hippocampus, a phenomenon that may well be related with the regulation of microtubule polymerization during post-lesion axonal growth.