Imaging Calcium in Neurons
Imaging Calcium in Neurons
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DOI：
10.1016/j.neuron.2012.02.011
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发表时间：
2012-03-08
期刊：
影响因子：
16.2
通讯作者：
Konnerth, Arthur
中科院分区：
文献类型：
--
作者：
Grienberger, Christine;Konnerth, Arthur
钙离子产生多种细胞内信号，控制所有类型神经元的关键功能。神经元中钙的成像特别重要，因为钙信号在明确的细胞亚室中发挥其高度特异性的功能。本文就神经元钙信号转导的一般机制作一综述。然后我们介绍了钙成像设备，包括共聚焦和双光子显微镜以及用于自由移动动物的小型化设备。我们提供了经典的化学荧光钙指示剂和基于蛋白质的遗传编码钙指示剂的概述。通过应用实例，我们介绍了该领域的新进展，例如清醒、行为动物的钙成像，以及在体内皮质神经元中使用钙成像来绘制单个脊柱感觉输入。最后，我们对钙成像在各种动物模型中分析神经元信号和可塑性的前景进行了展望。
Calcium ions generate versatile intracellular signals that control key functions in all types of neurons. Imaging calcium in neurons is particularly important because calcium signals exert their highly specific functions in well-defined cellular subcompartments. In this Primer, we briefly review the general mechanisms of neuronal calcium signaling. We then introduce the calcium imaging devices, including confocal and two-photon microscopy as well as miniaturized devices that are used in freely moving animals. We provide an overview of the classical chemical fluorescent calcium indicators and of the protein-based genetically encoded calcium indicators. Using application examples, we introduce new developments in the field, such as calcium imaging in awake, behaving animals and the use of calcium imaging for mapping single spine sensory inputs in cortical neurons in vivo. We conclude by providing an outlook on the prospects of calcium imaging for the analysis of neuronal signaling and plasticity in various animal models.