Near-infrared-excited upconversion photodynamic therapy of extensively drug-resistant Acinetobacter baumannii based on lanthanide nanoparticles
Near-infrared-excited upconversion photodynamic therapy of extensively drug-resistant Acinetobacter baumannii based on lanthanide nanoparticles
复制标题
基于稀土纳米粒子的近红外激发上转换光动力治疗广泛耐药鲍曼不动杆菌
DOI：
10.1039/d0nr01073a
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发表时间：
2020-07-14
期刊：
影响因子：
6.7
通讯作者：
Chen, Zhuo
中科院分区：
文献类型：
--
作者：
Liu, Wenzhen;Zhang, Yuxiang;Chen, Zhuo
广泛耐药鲍曼不动杆菌（XDR-AB）由于其对人类的致命伤害及其在医院中的高传播率引起了相当大的关注。然而，目前的抗生素不仅体内抗感染效果差，而且往往具有较高的肾毒性和神经毒性。在此，我们报道了一种基于光敏剂（PS）负载的上转换纳米粒子（UCNPs, LiYF4:Yb/Er）的近红外（NIR）光激发抗菌光动力疗法（aPDT）有效治疗体内XDR-AB感染。这种纳米试剂具有强大的近红外触发UC发光和从UCNPs到负载PS的高效能量转移，从而允许近红外触发的活性氧（ROS）的产生，以破坏细菌细胞膜。该策略对XDR-AB具有较高的抗菌活性，在50 μ g mL(-1) UCNPs-PVP-RB剂量的980 nm激光照射（1 W cm(-2)）下，其活性下降4.72 log（10）。更重要的是，我们可以在小鼠模型中对深层组织（约5 mm） XDR-AB感染取得出色的治疗效果，而不会产生任何副作用。总之，这种nir激活的aPDT可能为治疗各种深层组织难治性感染开辟了新的途径。
Extensively drug-resistant Acinetobacter baumannii (XDR-AB) has raised considerable concerns due to its mortal damage to humans and its high transmission rate of infections in hospitals. However, current antibiotics not only show poor anti-infection effects in vivo but also frequently cause high nephrotoxicity and neurotoxicity. Herein, we report a near-infrared (NIR) light-initiated antimicrobial photodynamic therapy (aPDT) to effectively treat in vivo XDR-AB infections based on photosensitizer (PS) loaded upconversion nanoparticles (UCNPs, LiYF4:Yb/Er). Such nanoagents feature robust NIR triggered UC luminescence and high-efficiency energy transfer from UCNPs to the loaded PS, thereby allowing NIR-triggered generation of reactive oxygen species (ROS) for destroying the bacterial cell membrane. This strategy permits a high antibacterial activity against XDR-AB, resulting in a decline of 4.72 log(10) in viability at a dose of 50 mu g mL(-1) UCNPs-PVP-RB with 980 nm laser irradiation (1 W cm(-2)). More significantly, we can achieve excellent therapeutic efficacy against deep-tissue (about 5 mm) XDR-AB infections without causing any side effects in the murine model. In brief, such NIR-activated aPDT may open up new avenues for treating various deep-tissue intractable infections.