Quantitative analysis of hemoglobin oxygenation state of rat brain in situ by near-infrared spectrophotometry.
Quantitative analysis of hemoglobin oxygenation state of rat brain in situ by near-infrared spectrophotometry.
复制标题
近红外分光光度法原位定量分析大鼠脑血红蛋白氧合状态。
DOI：
10.1152/jappl.1988.64.2.796
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发表时间：
1988
影响因子：
3.3
通讯作者：
M. Tamura
中科院分区：
文献类型：
--
作者：
O. Hazeki;M. Tamura
用近红外区(700~900 nm)的光照射大鼠头，在不同条件下用透射光测量吸收光谱。小于780 nm的吸光度变化与脑组织中的血红蛋白有关，而大于780 nm的吸光度变化与血红蛋白和细胞色素氧化酶有关。用A_(700-1.20)A_(730)和A_(700-1.52)A_(730)的表达可定量监测大鼠头部氧合和总(氧加脱氧)血红蛋白含量的变化。组织中的氧合血红蛋白含量随着吸入气体中O2压的降低而降低。在10%O2下，大约50%的血红蛋白被脱氧。在缺氧条件下，总血红蛋白含量升高。吸入5%二氧化碳和静脉注射钙离子阻滞剂尼卡地平可增加大脑中血红蛋白的氧饱和度。通过测量近红外区的差值吸收光谱，证实了上述结论。
The light in the near-infrared region (700-900 nm) was illuminated on the rat head, and absorption spectra were measured with the transmitted light under various conditions. The absorbance changes less than 780 nm were attributable to hemoglobin in the brain tissue, whereas those greater than 780 nm were associated with both hemoglobin and cytochrome oxidase. The changes of oxy- and total (oxy- plus deoxy-) hemoglobin content in the rat head could be monitored quantitatively by expressions of delta A700--1.20 delta A730 and delta A700--1.52 delta A730, respectively. The oxyhemoglobin content in the tissue was decreased as the O2 tension in inspired gas was lowered. At 10% O2 approximately 50% of hemoglobin was deoxygenated. The total hemoglobin content was increased under anoxic conditions. Inhalation of 5% CO2 and intravenous injection of a Ca2+ blocker nicardipine increased the O2 saturation of hemoglobin in the brain. These conclusions were confirmed by measuring the difference absorption spectra in the near-infrared region.