REGULATION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS .2. INHIBITION OF PROTEIN SYNTHESIS AT LEVEL OF INITIATION DURING MITOSIS
REGULATION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS .2. INHIBITION OF PROTEIN SYNTHESIS AT LEVEL OF INITIATION DURING MITOSIS
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DOI：
10.1016/0022-2836(70)90091-4
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发表时间：
1970-01-01
影响因子：
5.6
通讯作者：
PENMAN, S
中科院分区：
文献类型：
--
作者：
FAN, H;PENMAN, S
用秋水仙胺阻滞于中期的中国仓鼠卵巢细胞研究了真核细胞有丝分裂过程中蛋白质合成速率的下降。有丝分裂细胞的蛋白质合成速率约为间期速率的30%。核糖体附着在信使RNA上，并以降低的速率启动多肽合成。在多肽合成开始后，核糖体以正常速率翻译。用低水平的放线菌酮降低翻译速率可将大部分单体核糖体动员成活性多聚核糖体。与多聚核糖体相关的信使RNA的量也在用放线菌酮处理的有丝分裂细胞中增加。有丝分裂过程中蛋白质合成的减少似乎是由于核糖体与信使RNA的连接速率降低和多肽合成的起始。
The decline in the rate of protein synthesis during mitosis in eukaryotic cells was studied using Chinese hamster ovary cells arrested in metaphase with Colcemid. The rate of protein synthesis in mitotic cells is approximately 30% of the interphase rate. Ribosomes become attached to messenger RNA and initiate polypeptide synthesis at a reduced rate. After initiation of polypeptide synthesis, ribosomes are translated at a normal rate. Reducing the rate of translation with low levels of cycloheximide mobilizes a large proportion of monomeric ribosomes into active polyribosomes. The amount of messenger RNA associated with polyribosomes is also increased in mitotic cells treated with cycloheximide. It appears that the reduced protein synthesis during mitosis results from a lowered rate of attachment of ribosomes to messenger RNA and initiation of polypeptide synthesis.