Decreased anxiety-like behavior in beta3 nicotinic receptor subunit knockout mice
Decreased anxiety-like behavior in beta3 nicotinic receptor subunit knockout mice
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DOI：
10.1016/j.pbb.2007.04.011
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发表时间：
2007-05-01
影响因子：
3.6
通讯作者：
Collins, Allan C.
中科院分区：
文献类型：
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作者：
Booker, T. K.;Butt, Christopher M.;Collins, Allan C.
尼古丁通过烟碱乙酰胆碱受体家族激发许多生理反应，包括焦虑的改变。研究表明，尼古丁对焦虑的影响可能会支持吸烟行为。我们以前报道过，缺乏β 3烟碱受体亚单位的小鼠在旷场竞技场中表现出增加的活动。旷场活动已被证明是焦虑和运动活动的复合物，这两种行为都被尼古丁改变。因此，我们试图区分β 3受体在焦虑和运动活动中的作用。在高架十字迷宫、黑/白色箱和镜室中检测焦虑行为。β 3无效突变小鼠表现出焦虑减少，在高架十字迷宫的开放臂上花费的时间比其野生型同窝小鼠多。在黑/白色箱或镜室中未观察到显著差异。在基线和暴露于压力后，β 3无效突变小鼠的应激激素皮质酮水平显著较高。在β 3无效突变小鼠中也观察到Y-迷宫中的运动活动增加，但仅在暴露于应激后。这些发现强烈表明，含β 3的烟碱受体影响焦虑，可能是吸烟行为持续的关键。(c)2007年由Elsevier Inc.出版
Nicotine, via a family of nicotinic acetylcholine receptors, elicits many physiological responses, including alterations in anxiety. Studies suggest that the effects of nicotine on anxiety may support smoking behaviors. We reported previously that mice lacking the beta3 nicotinic receptor subunit demonstrate increased activity in the open field arena. Open field activity has been shown to be a composite of anxiety and locomotor activity, behaviors that are both altered by nicotine. We therefore sought to differentiate the role(s) of beta3-containing receptors in anxiety and locomotor activity. Anxiety behaviors were examined in the elevated plus maze, the black/white box and the mirrored chamber. Beta3 null mutant mice demonstrated decreased anxiety with more time spent on the open arm of the elevated plus maze than their wildtype littermates. No significant differences were observed with the black/white box or the mirrored chamber. Levels of the stress hormone, corticosterone, were significantly higher in the beta3 null mutant mice at baseline and following exposure to stress. Increased locomotor activity in the Y-maze was also observed for the beta3 null mutant mice, but only following exposure to stress. These findings strongly suggest that beta3-containing nicotinic receptors influence anxiety and may be critical for the continuation of smoking behaviors. (c) 2007 Published by Elsevier Inc.