SiRNA-Mediated Flotillin-2 (Flot2) Downregulation Inhibits Cell Proliferation, Migration, and Invasion in Gastric Carcinoma Cells
SiRNA-Mediated Flotillin-2 (Flot2) Downregulation Inhibits Cell Proliferation, Migration, and Invasion in Gastric Carcinoma Cells
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SiRNA 介导的 Flotillin-2 (Flot2) 下调抑制胃癌细胞的细胞增殖、迁移和侵袭
DOI：
10.3727/096504014x13946737557031
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发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Peng, Xiaowei
中科院分区：
文献类型：
--
作者：
Cao, Ke;Xie, Dingfang;Peng, Xiaowei
flotillin （flolot）蛋白家族已被证明参与各种癌症的发生和进展。然而，Flot2在胃癌中的作用尚不清楚。本研究旨在探讨Flot2在胃癌中的临床意义及作用。90例胃癌样本及其匹配癌旁组织的组织芯片数据显示，在90例癌旁组织中，65例癌旁组织不表达Flot2, 25例癌旁组织低表达Flot2，其阳性表达率仅为38.5% (25/90)；而在90例胃癌中，Flot2无表达6例，低表达26例，中等表达28例，高表达30例，其阳性表达率为93.3%（84/90）。此外，Flot2的表达与组织学分级、浸润深度、淋巴结转移和TNM分期显著相关。此外，生存分析数据表明，Flot2蛋白表达是生存不良的独立预后因素。然后，使用Flot2特异性siRNA降低胃癌AGS和5GC7901细胞中Flot2的表达。强制下调Flot2可显著抑制胃癌细胞的增殖、迁移和侵袭。综上所述，本研究提示，在胃癌中，Flot2蛋白表达与肿瘤进展及不良预后显著相关，可能与其在胃癌细胞中调控细胞增殖、迁移和侵袭有关。
The flotillin (Flot) protein family has been demonstrated to be involved in the development and progression of various cancers. However, the role of Flot2 in gastric carcinomas remains unknown. The present study aimed to investigate the clinical significance and the role of Flot2 in gastric carcinomas. Data of tissue microarray including 90 cases of gastric carcinoma samples and their matched adjacent tissues showed that, among 90 cases of adjacent tissues, 65 cases showed no Flot2 expression, and 25 cases showed low expression of Flot2, and its positive expression rate was only 38.5% (25/90); however, among 90 cases of gastric carcinomas, 6 cases showed no Flot2 expression, 26 cases showed low Flot2 expression, 28 cases showed moderate expression of Flot2, and 30 cases showed high expression of Flot2, and its positive expression rate was 93.3% (84/90). Moreover, the Flot2 expression was significantly associated with the histological grade, depth of invasion, lymph node metastasis, and TNM stage. Furthermore, data of survival analysis suggested that Flot2 protein expression was an independent prognostic factor of poor survival. After that, Flot2-specific siRNA was used to decrease the Flot2 expression in gastric cancer AGS and 5GC7901 cells. Forced downregulation of Flot2 remarkably inhibited cellular proliferation, migration, and invasion in gastric carcinoma cells. In conclusion, the present study suggests that the Flot2 protein expression is significantly correlated with cancer progression and poor prognosis in gastric carcinomas, probably due to its role in the regulation of cell proliferation, migration, and invasion in gastric carcinoma cells.