Treatment of human chronic wounds with autologous extracellular matrix/stromal vascular fraction gel: A STROBE-compliant study.
Treatment of human chronic wounds with autologous extracellular matrix/stromal vascular fraction gel: A STROBE-compliant study.
复制标题
用自体细胞外基质/基质血管成分凝胶治疗人类慢性伤口：一项符合 STROBE 标准的研究
DOI：
10.1097/md.0000000000011667
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发表时间：
2018-08
期刊：
影响因子：
1.6
通讯作者：
Lu F
中科院分区：
文献类型：
--
作者：
Deng C;Wang L;Feng J;Lu F
干细胞治疗被认为是最有前途的治疗慢性伤口。细胞外基质/基质血管成分凝胶（ECM/SVF凝胶）是一种基于脂肪来源干细胞的细胞疗法，已在动物模型的实验伤口中显示出愈合潜力。然而，ECM/SVF凝胶对人类慢性伤口的影响尚未研究。本研究的目的是探讨ECM/SVF凝胶对人类慢性创面的治疗作用。制备了AutoblastECM/SVF凝胶，并将其用于临床慢性创面的治疗，以负压创面治疗作为阳性对照。测量每周伤口愈合率和组织学变化。ECM/SVF凝胶组每周平均伤口愈合率为34.55 ± 11.18%，而负压伤口治疗组为10.16±2.67%（P <0.001）。 与对照组相比，用苏木精和伊红、Masson三色染色和CD 31免疫组织化学进行的组织学分析显示，在ECM/SVF凝胶组处理的皮肤中，淋巴细胞浸润较少，胶原积累较多，并且新形成的血管较多。ECM/SVF凝胶是临床慢性创面愈合的有效治疗选择。
Stem cell therapy is considered as the most promising treatment for chronic wounds. Extracellular matrix/stromal vascular fraction gel (ECM/SVF gel), an adipose-derived stem cell-based cytotherapy, has shown healing potential in experimental wounds in animal models. However, the effects of ECM/SVF gel on human chronic wounds have not been investigated. The aim of the present study is to investigate the therapeutic effect of ECM/SVF gel on human chronic wounds. Autologous ECM/SVF gel was prepared and used to treat patients with chronic wounds in clinics, with negative pressure wound therapy as the positive control. Wound healing rate per week and histological changes were performed. The average wound healing rate per week in the ECM/SVF gel group was 34.55 ± 11.18% compared with 10.16 ± 2.67% in the negative pressure wound therapy group (P < .001). Histological analysis with hematoxylin and eosin, Masson's trichrome staining, and CD31 immunohistochemistry showed less lymphocyte infiltration, more collagen accumulation, and more newly formed vessels in the ECM/SVF gel group treated skins compared to the control. ECM/SVF gel is an effective therapeutic option for chronic wound healing in clinics.