RESTORATION OF VISUAL CORTICAL PLASTICITY BY LOCAL MICROPERFUSION OF NOREPINEPHRINE
RESTORATION OF VISUAL CORTICAL PLASTICITY BY LOCAL MICROPERFUSION OF NOREPINEPHRINE
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DOI：
10.1002/cne.901850110
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发表时间：
1979-01-01
影响因子：
2.5
通讯作者：
ARY, M
中科院分区：
文献类型：
--
作者：
KASAMATSU, T;PETTIGREW, JD;ARY, M
使用新开发的连续微灌注技术，获得了进一步的证据来支持新皮质儿茶酚胺（CA），特别是去甲肾上腺素（NE），负责高水平皮质可塑性的假设。视觉皮层的变化被用于短暂的单眼剥夺后的眼优势，作为皮层可塑性的简单而可靠的指标。用 1 mg/ml (4.0 mM) 6-羟基多巴胺 (6-OHDA) 局部灌注小猫视觉皮层可防止小猫单眼剥夺的影响，从而复制使用脑室内注射获得的结果 (Kasamatsu 和 Pettigrew 76b, 79)。浓度为10-2 mg/ml (48.6 μM)的局部灌注NE恢复了不再对短暂的单眼睑缝合敏感的动物的视觉皮层可塑性。这些数字是指插管/微型泵系统中溶液的浓度。记录位置（约 2 毫米外）的有效浓度可能远低于这些浓度。 NE 灌注的这种效应在之前接受过 6-OHDA 治疗的小猫和已经过了敏感期的老年动物中都可见。在小猫中，眼的优势几乎完全转向睁开的眼睛，而在年长的动物中，双眼的视力下降。这些变化仅出现在灌注 NE 或 6-OHDA 的视觉皮层局部区域，而同一动物的附近皮层区域不受影响。除目视性外，单个神经元的感受野特性没有明显变化，并且外部灌注 NE 本身不会降低正常动物的双眼视度：上述 NE 的影响仅在动物的视觉体验同时改变时才会发生。 NE 在皮质可塑性中起着重要作用。
Using a newly developed technique of continuous microperfusion, further evidence was obtained in support of the hypothesis that the neocortical catecholamines (CA), particularly norepinephrine (NE), are responsible for a high level of cortical plasticity. The visual cortical changes were used in ocular dominance which follow a brief monocular deprivation as a simple and reliable index of cortical plasticity. Local perfusion of kitten visual cortex with 1 mg/ml (4.0 mM) 6-hydroxydopamine (6-OHDA) prevented the effects of monocular deprivation in kittens, thus replicating the results obtained using intraventricular injections (Kasamatsu and Pettigrew 76b, 79). Locally perfused NE at a concentration of 10-2 mg/ml (48.6 .mu.M) restored visual cortical plasticity in animals which were no longer susceptible to brief monocular lid-suture. These numbers refer to the concentration of solutions in the cannula/minipump system. The effective concentrations at the site of recording (about 2 mm away) are probably much lower than these. This effect of NE perfusion was seen both in kittens which had received prior 6-OHDA treatment and in older animals which had outgrown the susceptible period. In the kittens a nearly complete shift in ocular dominance was obtained toward the open eye and in the older animals a decrease in binocularity was obtained. The changes were found only in the local region of visual cortex perfused with either NE or 6-OHDA, while nearby cortical regions in the same animals were unaffected. There were no obvious changes in receptive field properties of individual neurons other than ocularity, and externally perfused NE did not itself reduce binocularity in normal animals: the effects of NE described above only occurred when the animal''s visual experience was simultaneously altered. NE plays an important role in cortical plasticity.