Effects of cationic liposomes with stearylamine against virus infection
Effects of cationic liposomes with stearylamine against virus infection
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DOI：
10.1016/j.ijpharm.2018.04.001
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发表时间：
2018-05
影响因子：
5.8
通讯作者：
K. Tahara;Manami Kobayashi;S. Yoshida;R. Onodera;N. Inoue;H. Takeuchi
中科院分区：
文献类型：
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作者：
K. Tahara;Manami Kobayashi;S. Yoshida;R. Onodera;N. Inoue;H. Takeuchi
在这项研究中，我们证明了阳离子脂质体与纳入硬脂胺（SA）抑制病毒感染，而无需预装活性药物成分。具体而言，我们使用基于重组杆状病毒（BV）的BacMam™试剂将脂质体的理化性质（如zeta电位和粒度）与病毒感染性相关联。与中性或带负电荷的脂质体相比，SA脂质体抑制BV在几种哺乳动物细胞系中的感染性，包括A549细胞。SA脂质体通过优化脂质体浓度和与细胞的接触时间抑制BV感染超过80%。此外，这些抗病毒SA脂质体没有细胞毒性，降低嵌入的胆固醇含量增强了抗病毒作用，同时增加了SA脂质体与细胞膜的结合。这些数据表明SA脂质体与细胞膜的结合可以阻断病毒进入。最后，我们还证明了SA脂质体在A549细胞中对单纯疱疹病毒1型的抗病毒作用，并显示出与抗病毒药物阿昔洛韦相当的疗效。
In this study, we demonstrated that cationic liposomes with incorporated stearylamine (SA) inhibit viral infectivity without preloaded active pharmaceutical ingredients. Specifically, we correlated physiochemical properties of liposomes, such as zeta potentials and particle sizes, with virus infectivity using the BacMam™ reagent, which is based on recombinant baculovirus (BV). Compared with neutral or negatively-charged liposomes, SA liposomes suppressed BV infectivity in several mammalian cell lines, including A549 cells. SA liposomes inhibited BV infection over 80% by optimizing the liposomal concentration and exposure time with cells. Moreover, these antiviral SA liposomes were not cytotoxic, and reducing the embedded cholesterol contents intensified the antiviral effects and simultaneously increased the binding of SA liposomes to the cell membranes. These data indicate that binding of SA liposomes to cell membranes may block virus entry. Finally, we also demonstrated the antiviral effects of SA liposomes on herpes simplex virus type 1 in A549 cells, and showed comparable efficacy to that of the antiviral drug acyclovir.