Vacancy affinity capillary electrophoresis, a new method for measuring association constants
Vacancy affinity capillary electrophoresis, a new method for measuring association constants
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DOI：
10.1016/s0021-9673(97)00205-7
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发表时间：
1997-07-18
影响因子：
4.1
通讯作者：
Poppe, H
中科院分区：
文献类型：
--
作者：
Busch, MHA;Boelens, HFM;Poppe, H
介绍了一种测定缔合常数的新方法-空位亲和毛细管电泳法。毛细管中充满相互作用组分的混合物，并注入纯缓冲液。在一系列实验中，一种组分的浓度是固定的，另一种组分的浓度是变化的。毛细管中两种物质的存在导致大的背景信号。电泳图中会出现两个负峰。负峰的迁移时间反映了混合物中游离和结合组分的分数，并取决于缓冲液中的浓度。这种偏移可以用于构建结合曲线，从该结合曲线可以估计缔合参数。几乎整个系统都充满了相互作用的组分，因此由于壁吸附而造成的损失较少。(C)1997年Elsevier Science B.V.
A new method to determine association constants, the vacancy affinity capillary electrophoretic (VACE) method is described. The capillary is filled with a mixture of the interacting components and neat buffer is injected The concentration of one of the components is fixed, and the concentration of the other one is varied during a series of experiments. The presence of both species in the capillary causes a large background signal. Two negative peaks will appear in the electropherogram. The migration times of the negative peaks reflect the fractions of the free and bound components in the mixture, and depends on the concentration in the buffer. This shift can be used to construct a binding curve from which the association parameters can be estimated. Nearly the whole system is saturated with the interacting components, so that losses due to wall adsorption are less detrimental. (C) 1997 Elsevier Science B.V.