The hppA gene of Helicobacter pylori encodes the class C acid phosphatase precursor
The hppA gene of Helicobacter pylori encodes the class C acid phosphatase precursor
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幽门螺杆菌的 hppA 基因编码 C 类酸性磷酸酶前体
DOI：
10.1016/s0014-5793(02)03064-8
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发表时间：
2002
期刊：
影响因子：
3.5
通讯作者：
E. K. Jagusztyn
中科院分区：
文献类型：
--
作者：
R. Godlewska;J. Bujnicki;J. Ostrowski;E. K. Jagusztyn
用感染的人和免疫的兔血清筛选幽门螺杆菌基因组文库，鉴定出具有酸性磷酸酶活性的25 kDa蛋白细胞包膜（HppA）。酶活性通过使用H的全细胞蛋白制备物的特异性酶测定来证明。pylori菌株N6和大肠杆菌（pUWM 192）。HppA在pH5.6时具有最佳活性，并且能抵抗EDTA的抑制。生物信息学分析和两个推定的活性位点残基（D 73和D192）的定点突变提供了对HppA作为DDDD磷酸水解酶超家族成员的序列-结构-功能关系的进一步了解。
Screening of theHelicobacter pylorigenomic library with sera from infected humans and from immunized rabbits resulted in identification of the 25 kDa protein cell envelope (HppA) which exhibits acid phosphatase activity. Enzyme activity was demonstrated by specific enzymatic assays with whole‐cell protein preparations ofH. pyloristrain N6 and fromEscherichia colicarrying thehppAgene (pUWM192). HppA showed optimum activity at pH 5.6 and was resistant to inhibition by EDTA. Bioinformatics analysis and site‐directed mutagenesis of two putative active site residues (D73 and D192) provide further insight into the sequence–structure–function relationships of HppA as a member of the DDDD phosphohydrolase superfamily.
影响因子：
2.9
作者：
Morais, MC;Zhang, WH;Allen, KN
通讯作者：
Allen, KN