Two isoforms of chicken melanopsins show blue light sensitivity
Two isoforms of chicken melanopsins show blue light sensitivity
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DOI：
10.1016/j.febslet.2007.10.019
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发表时间：
2007-11
期刊：
影响因子：
3.5
通讯作者：
M. Torii;D. Kojima;T. Okano;A. Nakamura;A. Terakita;Y. Shichida;A. Wada;Y. Fukada
中科院分区：
文献类型：
--
作者：
M. Torii;D. Kojima;T. Okano;A. Nakamura;A. Terakita;Y. Shichida;A. Wada;Y. Fukada
黑视蛋白是脊椎动物的非视觉视蛋白，在哺乳动物视网膜中起昼夜光感受器的作用。在这里，我们发现了两种黑视素基因在鸡松果体中的表达，并确定了这两个基因衍生的五种异构体的存在。重组蛋白与11-cis-retinal的重组显示，这些黑视素蛋白异构体中至少有两种可以作为蓝敏感性色素，其最大吸收波长为476- 484 nm。这些值与从哺乳动物黑视素的生理和行为研究确定的作用光谱的最大灵敏度是一致的。本研究中发现的黑视素亚型可能具有松果体昼夜光感受器的功能。
Melanopsin is a vertebrate non-visual opsin and functions as a circadian photoreceptor in mammalian retinas. Here we found the expression of two kinds of melanopsin genes in the chicken pineal gland and identified the presence of five isoforms derived from these two genes. Reconstitution of the recombinant proteins with 11-cis-retinal revealed that at least two of these melanopsin protein isoforms can function as blue-sensitive photopigments with absorption maxima at 476–484nm. These values are consistent with maximal sensitivities of action spectra determined from the physiological and behavioral studies on mammalian melanopsins. The melanopsin isoforms found in this study may function as pineal circadian photoreceptors.