SUPPRESSION OF MUTATIONS IN ALKALINE PHOSPHATASE STRUCTURAL CISTRON OF E COLI
SUPPRESSION OF MUTATIONS IN ALKALINE PHOSPHATASE STRUCTURAL CISTRON OF E COLI
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DOI：
10.1073/pnas.48.7.1121
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发表时间：
1962-01-01
影响因子：
11.1
通讯作者：
SIDDIQI, O
中科院分区：
文献类型：
--
作者：
GAREN, A;SIDDIQI, O
材料与方法细菌菌株：在所有实验中，Hfr为E. coli菌株K10，F-为E. coli菌株WI.这些菌株的特征已在别处描述。3-6先前与C. Levinthal和E.是林书用于遗传杂交的选择标记是T+和L+（在缺乏苏氨酸和亮氨酸的培养基中生长的能力）、Sr（在0.1mg/ml链霉素存在下生长的能力）和M+（在缺乏甲硫氨酸的培养基中生长的能力）。媒体：用于培养用于碱性磷酸酶测定的再培养物的低磷酸盐培养基的组成如下：1.2 X 10-1 M tris缓冲液; 0.2%葡萄糖; 6.4 X 10-5 M KHPO 4; 8 X 10-2 M NaCl; 2 X 10 -2 M ICI; 2 X 10 -2 M NH 4Cl; 3 X 10-3 M Na 2SO 4。
Materials and Methods.-Bacterial strains: In all experiments, the Hfr was E. coli strain K10 and the F-was E. coli strain WI. The characteristics of these strains have been described elsewhere. 3-6 Several of the P-mutants were previously isolated in collaboration with C. Levinthal and E. Lin. The selective markers employed for the genetic crosses were T+ and L+(ability to grow in a medium lacking threonine and leucine), Sr (ability to grow in the presence of 0.1 mg/ml of streptomycin), and M+(ability to grow in a medium lacking methionine). Media: The composition of the low phosphate mediumused to preparecultures for alkaline phosphatase assays was as follows: 1.2 X 10-1 M tris buffer; 0.2% glucose; 6.4 X 10-5 M KH PO4; 8 X 10-2 M NaCl; 2 X 10-2 M ICI; 2 X 10-2 M NH4C1 3 X 10-3 M Na2S04-