Effects of gene deletion of the tissue inhibitor of the matrix metalloproteinase-type 1 (TIMP-1) on left ventricular geometry and function in mice
Effects of gene deletion of the tissue inhibitor of the matrix metalloproteinase-type 1 (TIMP-1) on left ventricular geometry and function in mice
复制标题
基质金属蛋白酶 1 型组织抑制剂 (TIMP-1) 基因缺失对小鼠左心室几何形状和功能的影响
DOI：
10.1006/jmcc.1999.1052
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发表时间：
2000-01-01
影响因子：
5
通讯作者：
Spinale, FG
中科院分区：
文献类型：
--
作者：
Roten, L;Nemoto, S;Spinale, FG
基质金属蛋白酶(MMPs)及其组织抑制因子(TIMPs)表达和活性的改变与多种疾病状态下的组织重塑有关。TIMP-1是一种特性较好的TIMP，它可以与活性MMPs结合并调节基质金属蛋白酶的激活过程，本研究的目的是确定TIMP-1基因的缺失是否会对W的几何形状和功能产生时间依赖性的影响，从而消除左室心肌中TIMP-1的表达。年龄匹配的TIMP-1基因缺失同胞鼠(129Sv)(TIMP-1基因敲除(TIMP-1KO)，n=10)和野生型小鼠(n=10)在1月龄和4月龄时进行超声心动图对比研究。术后4个月行左心室插管检查，取左室作组织形态计量学研究。LV舒张末容量和质量增加(分别为18+/-4和38+/-3%，P
Alterations in the expression and activity of the matrix metalloproteinases (MMPs) and the tissue inhibitors of the MMPs (TIMPs) have been implicated in tissue remodeling in a number of disease states. One of the better characterized TIMPs, TIMP-1, has been shown to bind to active MMPs and to regulate the MMP activational process, The goal of this study was to determine whether deletion of the TIMP-1 gene in mice, which in turn would remove TIMP-1 expression in LV myocardium, would produce time-dependent effects on W geometry and function. Age-matched sibling mice (129Sv) deficient in the TIMP-1 gene (TIMP-1 knock-out (TIMP-1 KO), n = 10) and wild-type mice (n = 10) underwent comparative echocardiographic studies at 1 and 4 months of age. LV catheterization studies were performed at 4 months and the LV harvested for histomorphometric studies. LV end-diastolic volume and mass increased (18 +/- 4 and 38 +/- 3% , respectively, P