Identification and mapping of quantitative trait loci for cold tolerance at the booting stage in a japonica rice near-isogenic line
Identification and mapping of quantitative trait loci for cold tolerance at the booting stage in a japonica rice near-isogenic line
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粳稻近等基因系孕穗期耐寒数量性状位点的鉴定与定位
DOI：
10.1016/j.plantsci.2007.12.003
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发表时间：
2008-03-01
期刊：
影响因子：
5.2
通讯作者：
Li, Zi-Chao
中科院分区：
文献类型：
--
作者：
Xu, Li-Ming;Zhou, Lei;Li, Zi-Chao
近等基因系（NILs）是定位数量性状基因座（QTL）和评价基因作用或互作的首选材料。以强耐冷粳稻地方品种昆明小白谷（KMXBG）为母本，与冷敏粳稻十和田（Towada）为父本回交，选育出孕穗期耐冷的近等基因系。选择BC 4F 5系作为亲本以构建BC 5 F分离群体，从该群体开发BC 5 F3系。根据2年2个地点的主穗结实率，对孕穗期的耐冷性进行了评价。采用LOD显著性阈值为3.0，基于混合线性模型的综合区间作图揭示了位于第1、4、5、10和11染色体上的8个QTL。它们暂定为qCTB-1-1、qCTB-4-1、qCTB-4-2、qCTB-5-1、qCTB-5-2、qCTB-10-1、qCTB-10-2和qCTB-1 -1 -1。耐冷性的所有等位基因均由KMXBG贡献，标记间距缩小到0.6- 5.6cM。QTL峰值与最近标记的遗传距离在0 ~ 1.3cM之间。单个QTL解释的方差在0.90 ~ 14.86%之间。在2个或2个以上的试验中检测到4个QTL，分别为qCTB-1-1、qCTB-4-1、qCTB-5-1、qCTB-5-2和qCTB-10-1。与以往研究结果的比较表明，这些QTL是可靠的。本研究为进一步克隆水稻孕穗期耐冷基因奠定了基础，也为深入研究水稻孕穗期耐冷机制提供了可能。(c)2007爱思唯尔爱尔兰有限公司保留所有权利。
Near-isogenic lines (NILs) are preferred materials for mapping quantitative trait loci (QTLs) and for evaluating gene action or interaction. We developed a set of NILs with cold tolerance at the booting stage by backcrossing the strongly cold-tolerant japonica landrace, Kunmingxiaobaigu (KMXBG) to cold-sensitive japonica cultivar, Towada, as the recipient parent. A BC4F5 line was selected as a parent to construct a BC5F, segregating population from which BC5F3 lines were developed. Cold tolerance at the booting stage was evaluated on the basis of spikelet fertility of main panicles over 2 years and two locations. Using a LOD significance threshold of 3.0, compositive interval mapping based on a mixed linear model revealed eight QTLs on chromosomes 1, 4, 5, 10 and 11. They were tentatively designated qCTB-1-1, qCTB-4-1, qCTB-4-2, qCTB-5-1, qCTB-5-2, qCTB-10-1, qCTB-10-2 and qCTB-11-1. All alleles for cold tolerance were contributed by KMXBG, and the marker intervals containing them were narrowed to 0.6-5.6 cM. Genetic distances between the peaks of the QTL and nearest markers varied from 0 to 1.3 cM. The variance explained by a single QTL ranged from 0.90 to 14.86%. Four QTLs, qCTB-1-1, qCTB-4-1, qCTB-5-1, qCTB-5-2 and qCTB-10-1, were detected in two or more trials. Comparisons with previous studies indicated that these QTLs were reliable. Our study sets a foundation for cloning cold-tolerance genes and provides opportunities to understand the mechanism of cold tolerance at the booting stage. (c) 2007 Elsevier Ireland Ltd. All rights reserved.