Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis.

Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis.
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DOI：
10.1007/978-1-0716-2221-6_20
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发表时间：
2022-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
通讯作者：
Toseland, Christopher P
Toseland, Christopher P
中科院分区：
其他
文献类型：
--
作者：
Dos Santos, Alia;Gough, Rosemarie E;Toseland, Christopher P

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超分辨率显微镜能够实现蛋白质的高精度定位。因此，有可能研究细胞核内蛋白质的空间组织，以了解它们的组织如何与调节和功能相关。在这里，我们提出了单分子定位显微镜和聚类分析的方法，我们涵盖样品制备，图像采集和数据分析。
Super-resolution microscopy enables the high-precision localization of proteins. Therefore, it is possible to investigate the spatial organization of proteins within the nucleus to understand how their organization relates to regulation and function. Here, we present methodology for single-molecule localization microscopy and cluster analysis where we cover sample preparation, image acquisition, and data analysis.